豚鼠迟发型超敏反应性胸腔渗出液的研究。II. 单核细胞与淋巴细胞在迁移活性方面的相互关系。
Studies on delayed hypersensitivity pleural exudates in guinea-pigs. II. The interrelationship of monocytic and lymphocytic cells with respect to migration activity.
作者信息
Yamamoto S, Dunn C J, Willoughby D A
出版信息
Immunology. 1976 Apr;30(4):513-9.
The in vitro migration activity of monocytic cells and the effect of lymphocytes in the delayed hypersensitivity (DH) reaction induced by intrapleural injection of PPD into FCA-sensitized guinea-pigs was investigated. The in vitro migration of exudate mononuclear cells was lower than that of comparable blood cells, and continued to decrease as the reaction progressed. An inverse relaationship was observed between the migration area and cell volume of mononuclear cells from both blood and exudate. Similar experiments were performed on the reversed passive Arthus (RPA) reaction in the pleural cavity of guinea-pigs. For 6-h exudate, the mononuclear cell migration exceeded that for blood mononuclear cells. However, the migration of mononuclear cells from 18-h exudates was markedly reduced. As with DH, an inverse relationship between the migration area and cell volume was observed with the mononuclear cells of RPA exudates. Following the addition of antigen, the migration of DH exudate mononuclear cells was inhibited whereas the exudate mononuclear cells of the RPA reaction remained unaffected. For DH, the inhibition of migration induced by antigen appeared to be related to the non-adherent cells of 6-h exudates, and both the adherent and non-adherent cells of 18-h exudates.
研究了单核细胞的体外迁移活性以及淋巴细胞在向弗氏完全佐剂致敏的豚鼠胸膜腔内注射结核菌素纯蛋白衍生物（PPD）所诱导的迟发型超敏反应（DH）中的作用。渗出液单核细胞的体外迁移低于相应的血细胞，并且随着反应的进展持续下降。观察到血液和渗出液中单核细胞的迁移面积与细胞体积之间呈负相关。在豚鼠胸膜腔的反向被动阿图斯反应（RPA）中进行了类似实验。对于6小时的渗出液，单核细胞迁移超过血液单核细胞。然而，18小时渗出液中单核细胞的迁移明显减少。与DH一样，RPA渗出液的单核细胞在迁移面积与细胞体积之间观察到负相关。加入抗原后，DH渗出液单核细胞的迁移受到抑制，而RPA反应的渗出液单核细胞不受影响。对于DH，抗原诱导的迁移抑制似乎与6小时渗出液的非贴壁细胞以及18小时渗出液的贴壁和非贴壁细胞有关。
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