Thiry E, Wellemans G, Limbourg B, Broes A, Pastoret P P
Faculty of Veterinary Medicine, University of Liège, Belgium.
Vet Rec. 1992 Apr 25;130(17):372-5. doi: 10.1136/vr.130.17.372.
Forty-three cattle seronegative to bovine herpesvirus-1 (BHV-1) were given from one to five intradermal injections of BHV-1 inactivated antigen at four-week intervals. This delayed hypersensitivity test was assessed by the increase in skin thickness. The activity of the antigen was assessed in five animals which had a previous natural BHV-1 infection with clinical signs and seroconversion. Anti-BHV-1 antibodies were detected by seroneutralisation and an enzyme-linked immunoassay. Only one animal showed a significant but slight increase in skin thickness after the first test, but it was negative after a second test. The animals remained seronegative after the first test. Seroconversion was identified in 11 of the 43 animals (25 per cent) submitted to repeated delayed hypersensitivity tests. Five of 37 animals seroconverted after only two tests. The serological response was transient in seven of 11 seroconverted calves. Repeated hypersensitivity tests were therefore able to induce a serological response in seronegative calves but the response was weak and often transient. The test must therefore be applied cautiously to seronegative animals.
43头对牛疱疹病毒1型（BHV - 1）血清学阴性的牛，每隔四周接受1至5次BHV - 1灭活抗原皮内注射。通过皮肤厚度增加来评估这种迟发型超敏反应试验。在5只曾有过自然BHV - 1感染且出现临床症状和血清学转换的动物中评估抗原活性。通过血清中和试验和酶联免疫吸附测定检测抗BHV - 1抗体。仅1只动物在首次试验后皮肤厚度有显著但轻微增加，但第二次试验后呈阴性。首次试验后动物仍为血清学阴性。在接受重复迟发型超敏反应试验的43只动物中，有11只（25%）出现血清学转换。37只动物中有5只仅在两次试验后就发生了血清学转换。11只血清学转换的犊牛中有7只的血清学反应是短暂的。因此，重复超敏反应试验能够在血清学阴性的犊牛中诱导血清学反应，但反应较弱且往往是短暂的。所以，必须谨慎地对血清学阴性动物应用该试验。
