Trybała E, Wiśniewski J
Department of Veterinary Microbiology, Academy of Agriculture and Technology, Olsztyn, Poland.
Zentralbl Veterinarmed B. 1993 Feb;40(1):73-7. doi: 10.1111/j.1439-0450.1993.tb00111.x.
The effect of the repeated delayed-type hypersensitivity (DTH) testing (an intradermal test--ID) on virus neutralizing (VN) antibody and DTH responses has been studied. Repeated intradermal injection of inactivated BHV-1 antigen elicited neither the virus neutralizing antibody nor positive skin reaction in cattle free from BHV-1 infection. On the other, hand, the same manipulation carried out in a herd infected with BHV-1 provoked seroconversion and/or positive DTH reaction in some of the animals without detectable pre-existing VN antibody. Cattle initially positive by VN and intradermal tests showed steady increase in antibody titre, and some of them developed little or no skin reaction following repeated DTH testing.
已经研究了重复迟发型超敏反应（DTH）检测（皮内试验——ID）对病毒中和（VN）抗体和DTH反应的影响。对未感染牛疱疹病毒1型（BHV-1）的牛重复皮内注射灭活的BHV-1抗原，既未引发病毒中和抗体，也未引发阳性皮肤反应。另一方面，在感染BHV-1的牛群中进行相同操作，在一些没有可检测到的预先存在的VN抗体的动物中引发了血清转化和/或阳性DTH反应。最初通过VN和皮内试验呈阳性的牛，抗体滴度稳步上升，其中一些牛在重复DTH检测后皮肤反应很少或没有。
