Wiśniewski J, Trybała E
Department of Veterinary Microbiology, Academy of Agriculture and Technology, Olsztyn, Poland.
Zentralbl Veterinarmed B. 1993 Feb;40(1):21-6.
An intradermal (ID) test (a delayed-type hypersensitivity test) was used for the diagnosis of BHV-1 infection in cattle. A threshold value for the test positive results was established based on the analysis of the agreement between the results of ID test and those of virus neutralization (VN24) assay. Linear regression analysis revealed no correlation between the intensity of the skin reaction (expressed as an increase in skin-fold thickness) and the VN24 antibody titre. The sensitivity and specificity of the ID test compared to VN24 one, were 86.6% and 98.3% respectively. Of the 487 cattle with an age of over 6 months, 25 (5.1%) reacted discordantly by ID and VN24 tests, i.e. 19 seropositive animals expressed little or no skin reaction, whereas 6 seronegative individuals were positive by ID. Neither of the calves, with passively acquired antibodies in contrast to experimentally infected ones, showed distinctive skin reaction.
采用皮内（ID）试验（迟发型超敏反应试验）诊断牛的BHV - 1感染。基于对ID试验结果与病毒中和（VN24）试验结果之间一致性的分析，确定了该试验阳性结果的阈值。线性回归分析表明，皮肤反应强度（以皮褶厚度增加表示）与VN24抗体滴度之间无相关性。与VN24试验相比，ID试验的敏感性和特异性分别为86.6%和98.3%。在487头6个月以上的牛中，有25头（5.1%）ID试验和VN24试验结果不一致，即19头血清阳性动物皮肤反应轻微或无反应，而6头血清阴性个体ID试验呈阳性。与实验感染的小牛不同，被动获得抗体的小牛均未表现出明显的皮肤反应。
