Metzner M, Hofmann W, Lessmann H W
Klinik für Klauentiere, Fortpflanzung und Haltungshygiene, Freien Universität Berlin.
Tierarztl Prax. 1993 Jun;21(3):189-91.
Investigations concerning an intradermal test for the diagnosis of BHV 1 infection were carried out in 221 cattle. In 16% of cases the result of the test was dependent on the time the test was read (48 or 72 h). Fifteen per cent of the animals older than 9 months showed no reaction to the intradermal test though humoral antibodies against BHV 1 were detectable by ELISA. Intradermal injection of test antigen resulted in seroconversion in seronegative cattle. The antibodies remained detectable in the ELISA for about 3 to 6 weeks.