Li Vigni R, Turano A, Colombi M, Rampinelli F, Barlati S, Bianchi U A
Institute of Microbiology, University of Brescia.
Eur J Gynaecol Oncol. 1992;13(3):236-40.
The viral typization in Human Papillomavirus (HPV) infections of the lower female genital tract is relevant both from the epidemiological and the clinical point of view. We have tested DNA from specimens obtained by guided biopsies on neoplastic and benign lesions, using single probes of the different virus types (6, 11, 16 and 18) by Dot-blot and Southern blot analysis. According to previous studies HPV 16 and 18 have been detected in neoplastic lesions, while 6 and 11 were more frequently found associated to condylomata. Negative specimens and intermediate were 36% of the total. In our experience both methods have shown same sensibility.
从流行病学和临床角度来看，女性下生殖道人乳头瘤病毒（HPV）感染中的病毒分型都具有重要意义。我们使用不同病毒类型（6、11、16和18型）的单探针，通过斑点印迹和Southern印迹分析，对通过引导活检从肿瘤性和良性病变获取的标本DNA进行了检测。根据先前的研究，在肿瘤性病变中检测到了HPV 16和18型，而6和11型则更频繁地与湿疣相关。阴性标本和中间型标本占总数的36%。根据我们的经验，这两种方法显示出相同的灵敏度。
