Tsutsumi H, Tachibe T, Matsuoka A, Tokoi K
CSK Research Park, Inc., Nagano, Japan.
Exp Anim. 1996 Jul;45(3):261-3. doi: 10.1538/expanim.45.261.
Many experimental techniques for bile collection have been investigated. Most have been used under conditions of anaesthesia, or early after biliary cannulation. These conditions affect both the volume and the components of the bile, and the liver metabolism. In order to solve these problems, we devised an experimental method in which a T-cannula was introduced into the common bile duct of beagles that had been trained to remain at rest on the experimental table. Using this method, all of the bile could be collected under conscious conditions without the influence of surgical invasion.
许多用于胆汁收集的实验技术已被研究。大多数技术是在麻醉条件下或胆管插管后早期使用的。这些条件会影响胆汁的体积和成分以及肝脏代谢。为了解决这些问题，我们设计了一种实验方法，即将T形插管插入经过训练能在实验台上保持静止的比格犬的胆总管中。使用这种方法，可以在清醒状态下收集所有胆汁，而不受手术侵袭的影响。
