Vornhagen R, Baur A, Jahn G, Hinderer W, Nebel-Schickel H, Horn J, Wolf H, Sonneborn H H
Biotest AG, Research Department, Dreiechenhain, FRG.
Infusionsther Transfusionsmed. 1992 Apr;19(2):73-5. doi: 10.1159/000222586.
DNA-fragments coding for a variety of different viral antigens have been cloned and expressed in E. coli. Selected purified recombinant antigens were used for detection of specific antibodies by the means of ELISA technique. This approach has been used for the development of four different ELISA's for the detection of HIV- and EBV-specific antibodies.
编码多种不同病毒抗原的DNA片段已被克隆并在大肠杆菌中表达。选择纯化的重组抗原,通过ELISA技术检测特异性抗体。这种方法已被用于开发四种不同的ELISA,用于检测HIV和EBV特异性抗体。