Isolation and characterization of Escherichia coli mutants blocked in production of membrane-derived oligosaccharides.
作者信息
Weissborn A C, Rumley M K, Kennedy E P
机构信息
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
出版信息
J Bacteriol. 1992 Jul;174(14):4856-9. doi: 10.1128/jb.174.14.4856-4859.1992.
Abstract
We report a new procedure for the facile selection of mutants of Escherichia coli that are blocked in the production of membrane-derived oligosaccharides. Four phenotypic classes were identified, including two with a novel array of characteristics. The mutations mapped to two genetic loci. Mutations in the mdoA region near 23 min are in two distinct genes, only one of which is needed for the membrane-localized glucosyltransferase that catalyzes the synthesis of the beta-1,2-glucan backbone of membrane-derived oligosaccharides. Another set of mutations mapped near 27 min closely linked to osmZ; these appear to be in the galU gene.
摘要
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本文引用的文献
1
The influence of cultural conditions on polysaccharide production by Aerobacter aerogenes.
J Gen Microbiol. 1953 Oct;9(2):174-89. doi: 10.1099/00221287-9-2-174.
2
Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1092-5. doi: 10.1073/pnas.79.4.1092.
3
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J Bacteriol. 1984 Mar;157(3):956-7. doi: 10.1128/jb.157.3.956-957.1984.
4
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J Biol Chem. 1984 Jul 10;259(13):8388-93.
5
The biosynthesis of membrane-derived oligosaccharides. A membrane-bound phosphoglycerol transferase.
J Biol Chem. 1983 Feb 25;258(4):2394-8.
6
Biosynthesis of membrane-derived oligosaccharides: a periplasmic phosphoglyceroltransferase.
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5513-7. doi: 10.1073/pnas.78.9.5513.
7
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J Biol Chem. 1984 Oct 25;259(20):12644-51.
8
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J Bacteriol. 1984 Dec;160(3):976-81. doi: 10.1128/jb.160.3.976-981.1984.
9
Induction of capsular polysaccharide synthesis by rho-fluorophenylalanine in Escherichia coli wild type and strains with altered phenylalanyl soluble ribonucleic acid synthetase.
J Bacteriol. 1967 Feb;93(2):584-91. doi: 10.1128/jb.93.2.584-591.1967.
10
Chemotaxis in bacteria.
Science. 1966 Aug 12;153(3737):708-16. doi: 10.1126/science.153.3737.708.
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