Barnett B C, Dolan A, Telford E A, Davison A J, McGeoch D J
Institute of Virology, University of Glasgow, U.K.
J Gen Virol. 1992 Aug;73 ( Pt 8):2167-71. doi: 10.1099/0022-1317-73-8-2167.
Comparative analysis of DNA sequences located between the coding regions of genes UL49 and UL50 of herpes simplex virus types 1 and 2 (HSV-1 and -2) has revealed a small open reading frame (ORF) of 91 and 87 codons respectively with the characteristics of a genuine protein-coding region. The predicted protein products are clearly related and exhibit features of membrane-inserted proteins, with potential N-proximal signal peptides and C-proximal membrane anchor regions. Counterparts are present in the other sequenced alphaherpesviruses, namely varicella-zoster virus (a previously undescribed gene, 9A) and equine herpesvirus type 1 (gene 10), in the betaherpesvirus human cytomegalovirus (gene UL73) and in the gammaherpesvirus Epstein-Barr virus (gene BLRF1). Therefore, we consider that this ORF represents an additional HSV gene (UL49A) with counterparts in all sequenced alpha-, beta- and gammaherpesviruses.
对1型和2型单纯疱疹病毒（HSV-1和HSV-2）基因UL49和UL50编码区之间的DNA序列进行比较分析，发现了一个分别由91和87个密码子组成的小开放阅读框（ORF），具有真正蛋白质编码区的特征。预测的蛋白质产物明显相关，并表现出膜插入蛋白的特征，具有潜在的N端信号肽和C端膜锚定区。在其他已测序的α疱疹病毒中也存在对应物，即水痘带状疱疹病毒（一个先前未描述的基因，9A）和1型马疱疹病毒（基因10），在β疱疹病毒人巨细胞病毒（基因UL73）和γ疱疹病毒爱泼斯坦-巴尔病毒（基因BLRF1）中也存在。因此，我们认为这个ORF代表了一个额外的HSV基因（UL49A），在所有已测序的α、β和γ疱疹病毒中都有对应物。
