Efstathiou S, Ho Y M, Hall S, Styles C J, Scott S D, Gompels U A
Department of Pathology, University of Cambridge, U.K.
J Gen Virol. 1990 Jun;71 ( Pt 6):1365-72. doi: 10.1099/0022-1317-71-6-1365.
Short nucleotide sequence analysis of seven restriction fragments of murine herpesvirus 68 (MHV-68) DNA has been undertaken and used to determine the overall genome organization and relatedness of this virus to other well characterized representatives of the alpha-, beta- and gammaherpesvirus subgroups. Nine genes have been identified which encode amino acid sequences with greater similarity to proteins of the gammaherpesvirus Epstein-Barr virus (EBV) than to the homologous products of the alphaherpesviruses varicella-zoster virus and herpes simples virus type 1 or the betaherpesvirus human cytomegalovirus. In addition, the genome organization of MHV-68 is shown to have an overall collinearity with that of the gammaherpesviruses EBV and herpesvirus saimiri. In common with these viruses, dinucleotide frequency analysis of MHV-68 coding sequences reveals a marked reduction in CpG dinucleotide frequency thus implicating a dividing cell population as the site of latency in vivo.
已对鼠疱疹病毒68（MHV - 68）DNA的七个限制性片段进行了短核苷酸序列分析，并用于确定该病毒的整体基因组组织以及它与α -、β - 和γ - 疱疹病毒亚组中其他特征明确的代表病毒的亲缘关系。已鉴定出九个基因，这些基因编码的氨基酸序列与γ - 疱疹病毒爱泼斯坦 - 巴尔病毒（EBV）的蛋白质的相似性高于与α - 疱疹病毒水痘 - 带状疱疹病毒和单纯疱疹病毒1型或β - 疱疹病毒人巨细胞病毒的同源产物的相似性。此外，MHV - 68的基因组组织显示与γ - 疱疹病毒EBV和猴疱疹病毒的基因组组织具有整体共线性。与这些病毒一样，对MHV - 68编码序列的二核苷酸频率分析显示，CpG二核苷酸频率显著降低，因此暗示分裂细胞群体是体内潜伏的部位。
