Hinrichs S, Smith L, Ghazvini S, Kirven K, Palladino H, Spaulding D
Department of Medical Pathology, University of California, Davis.
Mod Pathol. 1992 May;5(3):257-61.
Specific human papillomavirus (HPV) types have been shown to be associated with proliferative epithelial lesions with variable biological consequences in infected patients. Simultaneous infection by more than one HPV type has been infrequently reported, and its clinical significance is unknown. We have examined four biopsies of cervical and vulvar tissue, each with evidence of infection by two different HPVs. Using both in situ hybridization and immunohistochemical techniques, we determined the cellular distribution of the viral infections. Using biotinylated type-specific probes and stringent conditions we were able to demonstrate that in each case the two HPVs occupied distinct, non-overlapping foci within the lesions. The condylomatous tissues contained DNA from HPV types that are associated with high-grade neoplasia and invasive cancer (16 and 18), as well as types commonly associated with benign proliferative lesions. Immunohistochemical analysis of the lesions with antibody to bovine papillomavirus capsid antigen failed to detect HPV in regions shown by in situ hybridization to contain HPV 16 and 18 DNA, whereas type 6 and 11 infected areas were readily identified. These results provide indirect evidence of viral interference between HPV types and indicate that interference may limit the number of HPV types that produce active infections within a single cell.
特定的人乳头瘤病毒（HPV）类型已被证明与感染患者中具有不同生物学后果的增殖性上皮病变相关。关于多于一种HPV类型同时感染的报道很少见，其临床意义尚不清楚。我们检查了四份宫颈和外阴组织活检样本，每份样本都有两种不同HPV感染的证据。我们使用原位杂交和免疫组织化学技术，确定了病毒感染的细胞分布。使用生物素化的型特异性探针并在严格条件下，我们能够证明在每种情况下，两种HPV在病变内占据不同的、不重叠的病灶。湿疣组织含有与高级别瘤变和浸润性癌相关的HPV类型（16型和18型）的DNA，以及通常与良性增殖性病变相关的类型。用抗牛乳头瘤病毒衣壳抗原抗体对病变进行免疫组织化学分析，未能在原位杂交显示含有HPV 16和18 DNA的区域检测到HPV，而6型和11型感染区域很容易被识别。这些结果提供了HPV类型之间病毒干扰的间接证据，并表明干扰可能会限制在单个细胞内产生活跃感染的HPV类型数量。
