Kanno H, Wolfinbarger J B, Bloom M E
Laboratory of Persistent Viral Diseases, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, Montana 59840.
J Virol. 1992 Sep;66(9):5305-12. doi: 10.1128/JVI.66.9.5305-5312.1992.
We examined Aleutian mink disease parvovirus (ADV) mRNA expression in lymph nodes of adult mink infected with ADV by Northern (RNA) blot and in situ hybridization. In Northern blot analysis, ADV transcripts were detected in the poly(A) RNA fraction extracted from mesenteric lymph nodes of two of five mink 10 days after intraperitoneal inoculation with the virulent Utah I strain of ADV. In strand-specific in situ hybridization, ADV DNA and mRNA were detected in some macrophagelike cells located in the medullary sinus in mesenteric lymph node sections from two of six infected mink by using biotinylated probes. In suspensions of lymph node cells, about 30% of the cells phagocytic for latex particles contained ADV DNA and about 1% of these cells contained ADV mRNA. In peritoneal exudate cells, about 20% of the macrophagelike cells contained ADV DNA and about 2% of these cells contained ADV mRNA. These results indicated that some macrophages in ADV-infected mink contained ADV mRNA and were target cells in ADV infection.
我们通过Northern（RNA）印迹法和原位杂交技术，检测了感染阿留申水貂病细小病毒（ADV）的成年水貂淋巴结中ADV的mRNA表达情况。在Northern印迹分析中，腹腔接种强毒株犹他I型ADV 10天后，在五只水貂中，有两只水貂肠系膜淋巴结提取的poly（A）RNA组分中检测到了ADV转录本。在链特异性原位杂交中，使用生物素化探针，在六只感染水貂中，有两只水貂肠系膜淋巴结切片髓窦中的一些巨噬样细胞中检测到了ADV DNA和mRNA。在淋巴结细胞悬液中，约30%吞噬乳胶颗粒的细胞含有ADV DNA，其中约1%的细胞含有ADV mRNA。在腹腔渗出细胞中，约20%的巨噬样细胞含有ADV DNA，其中约2%的细胞含有ADV mRNA。这些结果表明，ADV感染的水貂中的一些巨噬细胞含有ADV mRNA，是ADV感染的靶细胞。
