Koike K, Matsumoto K, Arai M, Yorifuji H, Fukutake K, Fujimaki M
Department of Clinical Pathology, Tokyo Medical College, Japan.
Int J Hematol. 1992 Apr;55(2):205-10.
We analyzed platelet-associated antigens from a hemophilia B patient with human immunodeficiency virus type 1 (HIV-1)-related thrombocytopenia. Two bands appeared at 31,000 and 37,000 daltons in the platelet lysate after reaction with autologous serum in SDS-PAGE and Western blots. The band at 37,000 daltons was obtained using anti-herpes simplex type 1 (HSV-1) rabbit antiserum. Doublet bands at 36,000 and 37,000 daltons also appeared after reaction with HSV-1 seropositive human serum. The band at 31,000 daltons appeared after reaction with anti-HIV-1 rabbit serum. These results suggest that the platelet-associated antigens in this patient are components of both HSV-1 and HIV-1 antigens. In addition, acyclovir decreased his PAIgG level and increased his platelet count, and zidovudine increased his platelet count. Thus, we concluded that each of the platelet-associated antigens is partially responsible for the thrombocytopenia by causing deposition of immune complexes in this patient.
我们分析了一名患有1型人类免疫缺陷病毒（HIV-1）相关血小板减少症的B型血友病患者的血小板相关抗原。在SDS-PAGE和蛋白质印迹法中，血小板裂解物与自体血清反应后，在31,000和37,000道尔顿处出现了两条条带。使用抗1型单纯疱疹病毒（HSV-1）兔抗血清获得了37,000道尔顿处的条带。与HSV-1血清阳性的人血清反应后，在36,000和37,000道尔顿处也出现了双峰条带。与抗HIV-1兔血清反应后，在31,000道尔顿处出现了条带。这些结果表明，该患者的血小板相关抗原是HSV-1和HIV-1抗原的成分。此外，阿昔洛韦降低了他的PAIgG水平并增加了他的血小板计数，齐多夫定增加了他的血小板计数。因此，我们得出结论，每种血小板相关抗原通过在该患者中引起免疫复合物沉积而部分导致血小板减少症。
