Ichikawa H, Helke C J
Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
Brain Res. 1992 Apr 24;578(1-2):311-6. doi: 10.1016/0006-8993(92)90263-9.
Cytochrome oxidase (CO) activity, an endogenous metabolic marker, was examined in visceral sensory neurons of the rat nodose and petrosal ganglia by using enzyme histochemistry. In the normal nodose and petrosal ganglia, nerve cells showed various degrees of staining intensity. The population of darkly stained neurons in the nodose ganglion was higher than in the petrosal ganglion. Axotomy of the peripheral axons of these bipolar sensory neurons was used to study potential changes in ganglionic cellular metabolism associated with loss of afferent inputs and/or injury. Peripheral axotomy had a significant effect on CO activity in the nodose ganglion. By 3 days after axotomy, darkly stained neurons decreased in number and lightly stained neurons, which were not observed in the normal ganglion, appeared in the nodose ganglion. At 7 days after axotomy, the average population of these lightly stained neurons increased to 29% in the nodose ganglion. Subsequently, the population decreased so that at 14 days and 21 days, 19% and 7% respectively of neurons were stained lightly. Even at 28 days after axotomy, the lightly stained neurons were still observed. In the petrosal ganglion, no remarkable change was observed at any stage after axotomy. These results suggest that metabolic activity decreases in some nodose neurons after peripheral nerve section.
细胞色素氧化酶（CO）活性是一种内源性代谢标志物，通过酶组织化学方法在大鼠结状神经节和岩神经节的内脏感觉神经元中进行检测。在正常的结状神经节和岩神经节中，神经细胞呈现出不同程度的染色强度。结状神经节中深色染色神经元的数量高于岩神经节。对这些双极感觉神经元的外周轴突进行切断，以研究与传入输入丧失和/或损伤相关的神经节细胞代谢的潜在变化。外周轴突切断对结状神经节中的CO活性有显著影响。轴突切断后3天，深色染色神经元数量减少，而正常神经节中未观察到的浅色染色神经元出现在结状神经节中。轴突切断后7天，结状神经节中这些浅色染色神经元的平均数量增加到29%。随后，该数量减少，以至于在14天和21天时，分别有19%和7%的神经元染色较浅。即使在轴突切断后28天，仍可观察到浅色染色神经元。在岩神经节中，轴突切断后的任何阶段均未观察到明显变化。这些结果表明，外周神经切断后，一些结状神经元的代谢活性降低。
