鞣酸对胃H⁺，K⁺-ATP酶的抑制作用。

Inhibitory effect of tannic acid on gastric H+,K(+)-ATPase.

作者信息

Murakami S, Muramatsu M, Otomo S

机构信息

Research Center, Taisho Pharmaceutical Co. Ltd., Saitama, Japan.

出版信息

J Nat Prod. 1992 Apr;55(4):513-6. doi: 10.1021/np50082a022.

DOI:10.1021/np50082a022

PMID:1324983

Abstract

The effect of tannic acid on gastric H+,K(+)-ATPase was studied. Tannic acid dose-dependently inhibited pig gastric H+,K(+)-ATPase activity with an IC50 value of 2.9 x 10(-8) M. Tannic acid also inhibited K(+)-stimulated p-nitrophenyl phosphatase (K(+)-pNPPase) activity, which is found in gastric H+,K(+)-ATPase preparations, as well as H+,K(+)-ATPase activity, with an IC50 value of 4.1 x 10(-7) M. Kinetic studies showed that the inhibition of H+,K(+)-ATPase activity by tannic acid was competitive with respect to ATP and noncompetitive with respect to K+. These results show that tannic acid is a potent inhibitor of gastric H+,K(+)-ATPase; this may be related to its anti-secretory and anti-ulcerogenic effects.

摘要

研究了鞣酸对胃H⁺，K⁺-ATP酶的作用。鞣酸剂量依赖性地抑制猪胃H⁺，K⁺-ATP酶活性，IC50值为2.9×10⁻⁸M。鞣酸还抑制胃H⁺，K⁺-ATP酶制剂中存在的K⁺刺激的对硝基苯磷酸酶（K⁺-pNPPase）活性以及H⁺，K⁺-ATP酶活性，IC50值为4.1×10⁻⁷M。动力学研究表明，鞣酸对H⁺，K⁺-ATP酶活性的抑制作用在ATP方面是竞争性的，在K⁺方面是非竞争性的。这些结果表明鞣酸是胃H⁺，K⁺-ATP酶的有效抑制剂；这可能与其抗分泌和抗溃疡作用有关。