Adefisayo Modinat A, Akomolafe Rufus O, Akinsomisoye Stephen O, Alabi Quadri K, Ogundipe Olaofe L, Omole Joseph G, Olamilosoye Kehinde P
Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo State, Nigeria.
Department of Physiological Sciences, Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
Toxicol Rep. 2017 Nov 13;4:625-633. doi: 10.1016/j.toxrep.2017.11.004. eCollection 2017.
This study investigated the protective effects of methanol extract of leaf (MEVA) on aspirin induced gastric ulcer in rats. Thirty Wistar rats, 150-200 g were divided into six groups as follows: Group 1 (control) rats received 2 mL/kg of propylene glycol for 28 consecutive days. Group 2 (Ulcer Control) received 150 mg/kg/day of aspirin suspended in 3 mL of 1% carboxymethylcellulose in water orally for 3 consecutive days during which the rats were fasted for the induction of ulcer. Group 3 received cimetidine at 100 mg/kg/day orally for 28 consecutive days and thereafter treated as group 2. Groups 4, 5 and 6 received MEVA orally at 200, 300 and 400 mg/kg/day respectively for 28 consecutive days and thereafter were treated with aspirin as group 2. All the animals were sacrifice at the end of the study to determine the gastric pH, gastric acidity, gastric ulcer score, haematological indices, superoxide dismutase (SOD) activity, reduced glutathione (GSH) and Lipid peroxidation (LPO) levels. The result showed that aspirin significantly (p < 0.05) increased gastric ulcer score and index, decreased gastric pH, gastric acidity, SOD activity, GSH level as well as increased LPO level. It induced significant necrosis of the stomach tissue. Administration of MEVA significantly (p < 0.05) increased gastric pH, but decreased gastric acid secretion and reversed alteration of haematological parameters. It also significantly (p < 0.05) increased SOD activity, GSH level and decreased LPO level. The results suggest that possesses gastro-protective properties against aspirin-induced gastric ulcer.
本研究调查了叶甲醇提取物（MEVA）对阿司匹林诱导的大鼠胃溃疡的保护作用。将30只体重150 - 200克的Wistar大鼠分为六组，如下：第1组（对照组）大鼠连续28天接受2毫升/千克丙二醇。第2组（溃疡对照组）在连续3天内每天口服150毫克/千克悬浮于3毫升1%羧甲基纤维素水溶液中的阿司匹林，在此期间大鼠禁食以诱导溃疡。第3组连续28天每天口服100毫克/千克西咪替丁，之后按第2组处理。第4、5和6组连续28天分别每天口服200、300和400毫克/千克MEVA，之后按第2组用阿司匹林处理。在研究结束时处死所有动物，以测定胃pH值、胃酸度、胃溃疡评分、血液学指标、超氧化物歧化酶（SOD）活性、还原型谷胱甘肽（GSH）和脂质过氧化（LPO）水平。结果表明，阿司匹林显著（p < 0.05）增加胃溃疡评分和指数，降低胃pH值、胃酸度、SOD活性、GSH水平以及增加LPO水平。它导致胃组织明显坏死。给予MEVA显著（p < 0.05）增加胃pH值，但减少胃酸分泌并逆转血液学参数的改变。它还显著（p < 0.05）增加SOD活性、GSH水平并降低LPO水平。结果表明，MEVA对阿司匹林诱导的胃溃疡具有胃保护特性。
