Hamaguchi Y, Oshiro I, Maeda J
Research & Development Division, Toa Medical Electronics Co., Ltd., Kobe.
Rinsho Byori. 1992 Jun;40(6):649-54.
The cyanmethemoglobin (HiCN) method has been adopted as the international standard procedure for hemoglobin (Hb) determinations due to the accuracy and stability of result. However, the presence of potassium cyanide (KCN) and potassium ferricyanide (K3-Fe(CN)6) in the reagents has raised problems of laboratory and environmental pollution. In 1981, Oshiro and colleagues developed a cyanide free method of Hb determination that is based on a low toxicity compound Sodium Lauryl Sulfate (SLS). The SLS-Hb method provides stable SLS-Hb formation through the following steps. 1) Reaction of SLS to erythrocytic membrane (disruption of the erythrocytic membrane). 2) Conformation change of Hb by SLS. 3) Iron oxidation by oxygen (Fe2+----Fe2+). 4) Formation of stable SLS-Hb (coordination of SLS). The paper presents several findings on the reaction mechanism of the SLS-Hb method.
由于结果的准确性和稳定性，氰化高铁血红蛋白（HiCN）法已被采用作为血红蛋白（Hb）测定的国际标准程序。然而，试剂中存在的氰化钾（KCN）和铁氰化钾（K3-Fe(CN)6）引发了实验室和环境污染问题。1981年，大岛及其同事开发了一种基于低毒化合物十二烷基硫酸钠（SLS）的无氰血红蛋白测定方法。SLS-Hb法通过以下步骤提供稳定的SLS-Hb形成。1）SLS与红细胞膜反应（红细胞膜破裂）。2）SLS引起Hb构象变化。3）氧气氧化铁（Fe2+----Fe2+）。4）形成稳定的SLS-Hb（SLS配位）。本文介绍了关于SLS-Hb法反应机制的若干发现。
