Karsan A, Maclaren I, Conn D, Wadsworth L
Department of Pathology, British Columbia Children's Hospital, Vancouver, Canada.
Am J Clin Pathol. 1993 Aug;100(2):123-6. doi: 10.1093/ajcp/100.2.123.
The standard reference method for hemoglobin determination for years has been the photometric quantitation of cyanmethemoglobin. Other existing methods have not gained popularity in North America. In an attempt to eliminate hazardous cyanide waste, a method to determine the hemoglobin concentration in whole blood using sodium lauryl sulfate has been devised and recently was released as a commercial reagent. The authors compare this new method with the cyanmethemoglobin method using two hematology analyzers. Their results show that there is excellent correlation between the two methods (r2 = 0.996). In addition, other elements of the complete blood count also show good correlation. Thus, it was confirmed that the sodium lauryl sulfate method of estimating hemoglobin concentration is an appropriate alternative to the cyanmethemoglobin method and avoids the generation of toxic wastes.
多年来，血红蛋白测定的标准参考方法一直是氰化高铁血红蛋白的光度定量法。其他现有方法在北美并未得到广泛应用。为了消除有害的氰化物废物，人们设计了一种使用十二烷基硫酸钠测定全血中血红蛋白浓度的方法，该方法最近作为一种商业试剂发布。作者使用两台血液分析仪将这种新方法与氰化高铁血红蛋白法进行了比较。他们的结果表明，两种方法之间具有极好的相关性（r2 = 0.996）。此外，全血细胞计数的其他指标也显示出良好的相关性。因此，已证实用十二烷基硫酸钠法估算血红蛋白浓度是氰化高铁血红蛋白法的一种合适替代方法，并且避免了有毒废物的产生。
