Gerna G, Sarasini A, Passarani N, Torsellini M, Parea M, Battaglia M
J Clin Microbiol. 1987 Jun;25(6):1137-9. doi: 10.1128/jcm.25.6.1137-1139.1987.
Using solid-phase immune electron microscopy (SPIEM) as a reference test, we examined 151 stool specimens from infants and young children with acute gastroenteritis for rotavirus detection by a one-step commercial enzyme-linked immunosorbent assay (ELISA) with labeled monoclonal antibody. Of the 83 samples determined to be positive for rotavirus by SPIEM, 82 were detected as positive by the monoclonal antibody ELISA (sensitivity, 98.7%), while 67 of the 68 specimens determined to be negative by SPIEM were correctly detected as negative by the ELISA (specificity, 98.5%). The diagnostic accuracy of the ELISA kit was 98.6%. Thus, the one-step monoclonal antibody ELISA, which can be completed in less than 90 min, appears to be highly suitable for the rapid and reliable detection of rotavirus in stools.
以固相免疫电子显微镜检查（SPIEM）作为参考检测方法，我们采用一种一步法商业性标记单克隆抗体酶联免疫吸附测定（ELISA），对151份急性胃肠炎婴幼儿粪便标本进行轮状病毒检测。在SPIEM检测确定为轮状病毒阳性的83份样本中，82份被单克隆抗体ELISA检测为阳性（灵敏度98.7%），而在SPIEM检测确定为阴性的68份标本中，67份被ELISA正确检测为阴性（特异性98.5%）。ELISA试剂盒的诊断准确性为98.6%。因此，可在不到90分钟内完成的一步法单克隆抗体ELISA似乎非常适合快速、可靠地检测粪便中的轮状病毒。
