用于粪便标本中轮状病毒检测的商用酶联免疫吸附测定法和固相免疫电子显微镜法的比较评估
Comparative evaluation of a commercial enzyme-linked immunoassay and solid-phase immune electron microscopy for rotavirus detection in stool specimens.
作者信息
Gerna G, Sarasini A, Passarani N, Torsellini M, Parea M, Battaglia M
出版信息
J Clin Microbiol. 1987 Jun;25(6):1137-9. doi: 10.1128/jcm.25.6.1137-1139.1987.
Abstract
Using solid-phase immune electron microscopy (SPIEM) as a reference test, we examined 151 stool specimens from infants and young children with acute gastroenteritis for rotavirus detection by a one-step commercial enzyme-linked immunosorbent assay (ELISA) with labeled monoclonal antibody. Of the 83 samples determined to be positive for rotavirus by SPIEM, 82 were detected as positive by the monoclonal antibody ELISA (sensitivity, 98.7%), while 67 of the 68 specimens determined to be negative by SPIEM were correctly detected as negative by the ELISA (specificity, 98.5%). The diagnostic accuracy of the ELISA kit was 98.6%. Thus, the one-step monoclonal antibody ELISA, which can be completed in less than 90 min, appears to be highly suitable for the rapid and reliable detection of rotavirus in stools.
摘要
以固相免疫电子显微镜检查（SPIEM）作为参考检测方法，我们采用一种一步法商业性标记单克隆抗体酶联免疫吸附测定（ELISA），对151份急性胃肠炎婴幼儿粪便标本进行轮状病毒检测。在SPIEM检测确定为轮状病毒阳性的83份样本中，82份被单克隆抗体ELISA检测为阳性（灵敏度98.7%），而在SPIEM检测确定为阴性的68份标本中，67份被ELISA正确检测为阴性（特异性98.5%）。ELISA试剂盒的诊断准确性为98.6%。因此，可在不到90分钟内完成的一步法单克隆抗体ELISA似乎非常适合快速、可靠地检测粪便中的轮状病毒。
相似文献
1
Comparative evaluation of a commercial enzyme-linked immunoassay and solid-phase immune electron microscopy for rotavirus detection in stool specimens.用于粪便标本中轮状病毒检测的商用酶联免疫吸附测定法和固相免疫电子显微镜法的比较评估
J Clin Microbiol. 1987 Jun;25(6):1137-9. doi: 10.1128/jcm.25.6.1137-1139.1987.
2
Diagnosis of acute non-bacterial gastroenteritis by rotavirus detection and serology.通过轮状病毒检测和血清学诊断急性非细菌性肠胃炎。
Microbiologica. 1984 Jan;7(1):29-39.
3
4
Rapid detection of human rotavirus strains in stools by single-sandwich enzyme-linked immunosorbent assay systems using monoclonal antibodies.使用单克隆抗体的夹心酶联免疫吸附测定系统快速检测粪便中的人轮状病毒株。
J Virol Methods. 1989 Apr-May;24(1-2):43-56. doi: 10.1016/0166-0934(89)90006-2.
5
Examination of the Rotazyme II enzyme immunoassay for the diagnosis of rotavirus gastroenteritis.用于诊断轮状病毒肠胃炎的Rotazyme II酶免疫测定法的检测
J Clin Microbiol. 1985 Sep;22(3):462-4. doi: 10.1128/jcm.22.3.462-464.1985.
6
Comparison of solid-phase immune electron microscopy by use of protein A with direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in stool.使用蛋白A的固相免疫电子显微镜与直接电子显微镜和酶联免疫吸附测定法在粪便中检测轮状病毒的比较。
J Clin Microbiol. 1983 Nov;18(5):1244-9. doi: 10.1128/jcm.18.5.1244-1249.1983.
7
Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children.用于检测儿童肠胃炎病毒的直接电子显微镜检查、免疫电子显微镜检查和轮状病毒酶联免疫吸附测定的比较
J Clin Microbiol. 1981 May;13(5):976-81. doi: 10.1128/jcm.13.5.976-981.1981.
8
Rapid diagnosis of rotavirus gastroenteritis by a commercial latex agglutination test.通过商业乳胶凝集试验快速诊断轮状病毒肠胃炎。
J Clin Microbiol. 1985 Nov;22(5):846-50. doi: 10.1128/jcm.22.5.846-850.1985.
9
Laboratory diagnosis of rotavirus infection in diarrhoeal patients by immunoenzymatic and latex-agglutination assays.通过免疫酶法和乳胶凝集试验对腹泻患者轮状病毒感染进行实验室诊断。
Microbiologica. 1992 Jul;15(3):249-57.
10
Comparison of solid-phase immune electron microscopy, direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in faecal samples.固相免疫电子显微镜、直接电子显微镜和酶联免疫吸附测定法检测粪便样本中轮状病毒的比较。
J Virol Methods. 1982 Feb;4(1):45-53. doi: 10.1016/0166-0934(82)90053-2.
引用本文的文献
1
[Rotavirus neonatal diarrhea].[轮状病毒新生儿腹泻]
Med Mal Infect. 1991 Oct;21:585-588. doi: 10.1016/S0399-077X(05)81180-1. Epub 2005 Jun 2.
2
Characterization of VP6 genes from rotavirus strains collected in the United States from 1996-2002.对1996年至2002年在美国收集的轮状病毒株的VP6基因进行的特征分析。
Virus Genes. 2007 Dec;35(3):489-95. doi: 10.1007/s11262-007-0119-7. Epub 2007 Jun 13.
3
Verification of sensitivity and specificity of group a rotavirus detection in piglets faeces with monoclonal blocking ELISA methods.用单克隆阻断ELISA方法验证仔猪粪便中A组轮状病毒检测的敏感性和特异性。
J Vet Med B Infect Dis Vet Public Health. 2004 May;51(4):160-5. doi: 10.1111/j.1439-0450.2004.00746.x.
4
Diagnostic electron microscopy is still a timely and rewarding method.诊断电子显微镜检查仍然是一种及时且有价值的方法。
J Clin Virol. 1999 Jun;13(1-2):105-19. doi: 10.1016/s1386-6532(99)00027-x.
5
Ability of TESTPACK ROTAVIRUS enzyme immunoassay to diagnose rotavirus gastroenteritis.TESTPACK轮状病毒酶免疫测定法诊断轮状病毒肠胃炎的能力。
J Clin Microbiol. 1988 Nov;26(11):2459-61. doi: 10.1128/jcm.26.11.2459-2461.1988.
6
Comparison of nine commercial immunoassays for the detection of rotavirus in fecal specimens.九种用于检测粪便标本中轮状病毒的商用免疫测定法的比较。
J Clin Microbiol. 1988 Sep;26(9):1630-4. doi: 10.1128/jcm.26.9.1630-1634.1988.
7
Human viral gastroenteritis.人类病毒性肠胃炎
Clin Microbiol Rev. 1989 Jan;2(1):51-89. doi: 10.1128/CMR.2.1.51.
8
Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains.轮状病毒亚组特异性单克隆抗体的特性及其在单夹心ELISA系统中用于人毒株快速亚组分型的应用
Arch Virol. 1989;107(3-4):315-22. doi: 10.1007/BF01317927.
9
Comparison of a new rapid test (TestPack Rotavirus) with standard enzyme immunoassay and electron microscopy for the detection of rotavirus in symptomatic hospitalized children.一种新型快速检测方法（TestPack轮状病毒检测试剂盒）与标准酶免疫测定法及电子显微镜检查法在有症状住院儿童中检测轮状病毒的比较。
J Clin Microbiol. 1989 Apr;27(4):775-7. doi: 10.1128/jcm.27.4.775-777.1989.
10
Comparison of five enzyme immunoassays, electron microscopy, and latex agglutination for detection of rotavirus in fecal specimens.五种酶免疫测定法、电子显微镜检查法和乳胶凝集法检测粪便标本中轮状病毒的比较
J Clin Microbiol. 1989 Feb;27(2):364-6. doi: 10.1128/jcm.27.2.364-366.1989.
本文引用的文献
1
Diagnosis of acute non-bacterial gastroenteritis by rotavirus detection and serology.通过轮状病毒检测和血清学诊断急性非细菌性肠胃炎。
Microbiologica. 1984 Jan;7(1):29-39.
2
Evaluation of enzyme immunoassays for the detection of human rotavirus.用于检测人轮状病毒的酶免疫测定法的评估
J Infect Dis. 1981 Oct;144(4):379. doi: 10.1093/infdis/144.4.379.
3
Comparison of immunosorbent electron microscopy, enzyme immunoassay and counterimmunoelectrophoresis for detection of human rotavirus in stools.免疫吸附电子显微镜法、酶免疫测定法和对流免疫电泳法检测粪便中人类轮状病毒的比较
J Virol Methods. 1981 Sep;3(2):99-107. doi: 10.1016/0166-0934(81)90006-9.
4
Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children.用于检测儿童肠胃炎病毒的直接电子显微镜检查、免疫电子显微镜检查和轮状病毒酶联免疫吸附测定的比较
J Clin Microbiol. 1981 May;13(5):976-81. doi: 10.1128/jcm.13.5.976-981.1981.
5
Subgrouping of human rotavirus strains by complement fixation, indirect double-antibody sandwich enzyme-linked immunosorbent assay and solid-phase immune electron microscopy.通过补体结合试验、间接双抗体夹心酶联免疫吸附测定和固相免疫电镜对人轮状病毒毒株进行亚群分类。
Arch Virol. 1984;81(3-4):193-203. doi: 10.1007/BF01309992.
6
Detection of rotavirus in human stools by using monoclonal antibody.利用单克隆抗体检测人粪便中的轮状病毒。
J Clin Microbiol. 1984 Jun;19(6):888-92. doi: 10.1128/jcm.19.6.888-892.1984.
7
Detection of rotavirus in stool specimens with monoclonal and polyclonal antibody-based assay systems.使用基于单克隆和多克隆抗体的检测系统检测粪便标本中的轮状病毒。
J Clin Microbiol. 1986 May;23(5):897-900. doi: 10.1128/jcm.23.5.897-900.1986.
8
Characterization of serotypes of human rotavirus strains by solid-phase immune electron microscopy.通过固相免疫电子显微镜对人轮状病毒株血清型进行鉴定。
J Infect Dis. 1985 Dec;152(6):1143-51. doi: 10.1093/infdis/152.6.1143.
9
Comparative efficiency of commercial immunoassays for the diagnosis of rotavirus gastroenteritis during the course of infection.商用免疫测定法在轮状病毒胃肠炎感染过程中用于诊断的比较效率
J Clin Microbiol. 1985 Nov;22(5):693-8. doi: 10.1128/jcm.22.5.693-698.1985.
10
Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis.用于检测婴幼儿肠胃炎人呼肠孤病毒样病原体的酶联免疫吸附测定（ELISA）
Lancet. 1977 Aug 6;2(8032):263-7. doi: 10.1016/s0140-6736(77)90951-5.
Zotero 插件