He K W, Lin J H, Ding Z D, He J H, Jiang J Y, Hou J B
Institute of Animal Husbandry and Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, People's Republic of China.
Zentralbl Veterinarmed B. 1992 Jun;39(4):299-302. doi: 10.1111/j.1439-0450.1992.tb01172.x.
Eight strains of rotaviruses isolated from diarrheal animals (4 from calves and 4 from piglets) in China were compared by serotyping with reference animal rotavirus strains (bovine NCDV, porcine OSU and simian SA-11 and human rotavirus Wa strain). Two-way cross neutralization test showed no antigenic difference between all 4 local strains of bovine rotavirus (BRV007, BRV014, HN-7 and BRV6555) and reference NCDV, so they belonged to rotavirus serotype 6 (bovine rotavirus serotype 1 or NCDV-serotype). Meanwhile, the four strains of Chinese porcine rotavirus could be determined into 2 different serotypes. One (Li99) was neutralised to a high titer with the antiserum against reference OSU virus and probably related to OSU (serotype 5 or porcine serotype 1). The other three strains (Lin71, Nan86 and Jiang150) were antigenically obviously different from Li99 and did not react with the antiserum against OSU. They were tentatively considered as porcine rotavirus serotype 2. All the strains of bovine and porcine rotavirus did not cross-neutralise with simian SA-11 and human Wa strain. There was also no antigenic relationship between bovine rotaviruses and porcine rotaviruses.
采用参考动物轮状病毒株（牛NCDV、猪OSU、猴SA - 11和人轮状病毒Wa株）对从中国腹泻动物（4头犊牛和4头仔猪）中分离出的8株轮状病毒进行血清分型比较。双向交叉中和试验表明，所有4株本地牛轮状病毒（BRV007、BRV014、HN - 7和BRV6555）与参考NCDV之间无抗原差异，因此它们属于轮状病毒血清型6（牛轮状病毒血清型1或NCDV血清型）。同时，4株中国猪轮状病毒可分为2种不同血清型。其中一株（Li99）被抗参考OSU病毒血清高滴度中和，可能与OSU相关（血清型5或猪血清型1）。另外3株（Lin71、Nan86和Jiang150）与Li99抗原性明显不同，且不与抗OSU血清反应。它们暂被认为是猪轮状病毒血清型2。所有牛和猪轮状病毒株均不与猴SA - 11和人Wa株交叉中和。牛轮状病毒和猪轮状病毒之间也不存在抗原关系。
