Hoshino Y, Wyatt R G, Greenberg H B, Kalica A R, Flores J, Kapikian A Z
Infect Immun. 1983 Sep;41(3):1031-7. doi: 10.1128/iai.41.3.1031-1037.1983.
A rotavirus designated strain H-2 was isolated in primary African green monkey kidney cells from a foal with diarrhea. This cell culture-adapted strain was found to be similar, if not identical, to simian rotavirus (strains MMU18006 and SA-11) and canine rotavirus (strain CU-1) and, in addition, demonstrated a one-way antigenic relationship with five human rotavirus strains (P, B, no. 14, no. 15, and YO) of the third human rotavirus serotype by the plaque reduction neutralization test. This is the fifth example of an animal rotavirus which shares serotypic specificity with a human rotavirus. The H-2 strain is distinct from the H-1 strain (Y. Hoshino et al., J. Clin. Microbiol., in press) of equine rotavirus not only in serotypic specificity by neutralization but also in subgroup specificity, hemagglutinating activity, and RNA electrophoretic migration pattern, thus establishing the existence of a second equine rotavirus serotype. This H-2 isolate is also distinct by neutralization from three other human rotavirus serotypes, 1 (Wa), 2 (DS-1), and 4 (St. Thomas no. 4), as well as bovine (NCDV), and porcine (OSU) rotaviruses.
从一匹患腹泻的小马驹体内分离出一株轮状病毒，命名为H - 2株。该病毒是在原代非洲绿猴肾细胞中分离得到的。经发现，这种适应细胞培养的毒株即便与猿猴轮状病毒（MMU18006株和SA - 11株）及犬轮状病毒（CU - 1株）不完全相同，至少也是相似的。此外，通过蚀斑减少中和试验表明，它与第三种人类轮状病毒血清型的五株人类轮状病毒毒株（P、B、14号、15号和YO）存在单向抗原关系。这是动物轮状病毒与人类轮状病毒具有血清型特异性相同的第五个实例。H - 2株与马轮状病毒H - 1株（Y. 星野等人，《临床微生物学杂志》，即将发表）不同，不仅在中和血清型特异性方面，而且在亚组特异性、血凝活性和RNA电泳迁移模式方面均有差异，从而确定存在第二种马轮状病毒血清型。通过中和试验，该H - 2分离株也与其他三种人类轮状病毒血清型（1型（Wa）、2型（DS - 1）和4型（圣托马斯4号））以及牛（NCDV）和猪（OSU）轮状病毒不同。
