Haukenes G, Brinchmann-Hansen K, Macovei O
Department of Microbiology and Immunology, Gade Institute, University of Bergen, Norway.
APMIS. 1992 Aug;100(8):757-61. doi: 10.1111/j.1699-0463.1992.tb03996.x.
Two hundred and fifteen children in an orphanage in Romania were examined for serum markers of present or past hepatitis B and C virus and HIV infection. In total, 183 children (85.1%) had at least one marker (HBsAg, anti-HBs or anti-HBc) of hepatitis B virus infection. An HBsAg carrier state was diagnosed in 38 (20.8%) of the infected children. Among the carriers 24.3% were HBeAg carriers, 51.4% had anti-HBe and 24.3% had neither HBe antigen nor antibody. Nine children (4.2%) had antibodies to hepatitis C virus. All sera were negative in tests for HIV antibodies. False-positive reactions represented a considerable problem with these sera. Six percent of the sera gave false-positive reactions in indirect ELISA tests for hepatitis C and HIV. Sera giving false-positive reactions had rather high serum IgG levels. The results of this study indicate that these children have been heavily exposed to hepatitis B virus and to a certain degree to hepatitis C virus, while there were no cases of HIV infection in this orphanage.
对罗马尼亚一家孤儿院的215名儿童进行了血清检测，以筛查当前或既往的乙肝病毒、丙肝病毒及艾滋病毒感染标志物。共有183名儿童（85.1%）至少有一项乙肝病毒感染标志物（乙肝表面抗原、乙肝表面抗体或乙肝核心抗体）。在这些受感染儿童中，38名（20.8%）被诊断为乙肝表面抗原携带者状态。在携带者中，24.3%为乙肝e抗原携带者，51.4%有乙肝e抗体，24.3%既无乙肝e抗原也无乙肝e抗体。9名儿童（4.2%）有丙肝病毒抗体。所有血清的艾滋病毒抗体检测均为阴性。假阳性反应在这些血清检测中是一个相当严重的问题。6%的血清在丙肝和艾滋病毒间接酶联免疫吸附试验中出现假阳性反应。出现假阳性反应的血清其血清免疫球蛋白G水平相当高。本研究结果表明，这些儿童曾大量接触乙肝病毒，在一定程度上也接触过丙肝病毒，而该孤儿院未发现艾滋病毒感染病例。
