[在兔实验性角膜炎中碘苷（IUdR）耐药性对单纯疱疹病毒1型（HSV1）毒株敏感性的可逆性]

[Reversibility of IUdR resistance to sensitivity to an HSV1 strain in experimental keratitis in rabbits].

作者信息

Fardeau C, Langlois M, Ingrand D, Asselot C, Moret H, Nugier F

机构信息

Unité de Recherches d'Ophtalmologie, I.N.S.E.R.M. U n. 86, Hôtel-Dieu, Paris.

出版信息

C R Acad Sci III. 1992;314(10):443-9.

PMID:1325862

Abstract

During 7 serial passages of Herpes Simplex Virus (HSV1) in rabbit cornea treated with idoxuridine (IUdR) (P1 to P7), the emergence of resistance had been obtained from P3. The reversion towards IUdR sensitivity has been investigated from either viral population P3 or P6 by 6 serial passages in rabbit cornea treated by Vaseline (V1 to V6). From viral population P3, the reversion to IUdR sensitivity has been obtained at V4. In contrast, from viral population P6, the IUdR resistance was conserved from V1 to V6. In vitro, on Vero cells, the effective doses 50% (ED50) and 90% (ED90), determined by dye uptake assay and plaque reduction assay, confirmed the reversibility towards IUdR sensitivity obtained from P3 and the stability of IUdR resistance from P6.

摘要

在用碘苷（IUdR）处理的兔角膜中，单纯疱疹病毒1型（HSV1）连续传代7次（P1至P7）期间，从P3开始出现了耐药性。通过在涂有凡士林的兔角膜中连续传代6次（V1至V6），研究了病毒群体P3或P6对IUdR敏感性的回复情况。从病毒群体P3开始，在V4时获得了对IUdR敏感性的回复。相比之下，从病毒群体P6开始，从V1到V6都保持了对IUdR的耐药性。在体外，在Vero细胞上，通过染料摄取试验和蚀斑减少试验确定的半数有效剂量（ED50）和90%有效剂量（ED90），证实了从P3获得的对IUdR敏感性的可逆性以及P6对IUdR耐药性的稳定性。