Rose B R, Thompson C H, Chantrill L A, Tattersall M H, Cossart Y E
Department of Infectious Diseases, University of Sydney, Australia.
Int J Cancer. 1992 Sep 9;52(2):225-8. doi: 10.1002/ijc.2910520212.
The polymerase chain reaction (PCR) was used to investigate the prevalence and distribution of human papillomavirus (HPV)-16 DNA in paraffin sections of all pelvic lymph nodes removed from 14 patients with Stage Ib-cervical cancer at the time of resection of their primary tumours. The results were compared with those obtained from 8 women with no known history of cervical abnormality. In all, 22 cervical biopsies and 40 I lymph nodes (296 paraffin blocks) were examined. Nine of the 14 cervical cancer patients had primary tumours that were positive for HPV-16 DNA: only 3 of these had lymph nodes with histological evidence of metastasis, and HPV 16 DNA was detected in each of the corresponding paraffin blocks. HPV 16 DNA was also detected in varying proportions (8%-92%) of the histologically-negative lymph nodes from these women. There was no correlation between the HPV DNA-positive lymph nodes and their proximity to the primary tumour. HPV-16 DNA was not identified in any of the lymph nodes from the 5 women whose cancers were not HPV-16-related, or in those of women with no evidence of cervical abnormality. This preliminary survey suggests that HPV DNA is frequently transported from HPV-16-related cervical tumours to regional lymph nodes. However, its practical significance will not be clear until sufficient time has elapsed for correlation of the results with the clinical outcome.
采用聚合酶链反应（PCR）研究14例Ib期宫颈癌患者在原发肿瘤切除时所切除的所有盆腔淋巴结石蜡切片中人乳头瘤病毒（HPV）-16 DNA的流行情况和分布。将结果与8例无已知宫颈异常病史的女性的结果进行比较。总共检查了22份宫颈活检标本和40个I级淋巴结（296个石蜡块）。14例宫颈癌患者中有9例原发肿瘤HPV-16 DNA呈阳性：其中只有3例有淋巴结转移的组织学证据，并且在每个相应的石蜡块中均检测到HPV 16 DNA。在这些女性组织学阴性的淋巴结中也检测到不同比例（8%-92%）的HPV 16 DNA。HPV DNA阳性的淋巴结与其与原发肿瘤的距离之间没有相关性。在5例癌症与HPV-16无关的女性的任何淋巴结中，或在无宫颈异常证据的女性的淋巴结中均未鉴定出HPV-16 DNA。这项初步调查表明，HPV DNA经常从与HPV-16相关的宫颈肿瘤转移至区域淋巴结。然而，在有足够时间将结果与临床结局进行关联之前，其实际意义尚不清楚。
