Plata F, Autran B, Martins L P, Wain-Hobson S, Raphaël M, Mayaud C, Denis M, Guillon J M, Debré P
Nature. 1987;328(6128):348-51. doi: 10.1038/328348a0.
Human immunodeficiency virus (HIV) is implicated in the development of AIDS (acquired immune deficiency syndrome). HIV infection leads to the generation of HIV-specific thymus-derived (T) lymphocytes in humans and apes. We describe an experimental system permitting the quantitative and systematic analysis of HIV-specific cytotoxic T lymphocytes (CTL). Functional, HIV-specific CTL are obtained by broncho-alveolar lavage (BAL) from the lungs of seropositive patients with lymphocytic alveolitis. These alveolar CTL: (1) recognize and kill HIV-infected alveolar macrophages in vitro under autologous, but not heterologous, conditions; (2) correspond to standard CTL as they express the CD3 and CD8 surface markers, but not the CD4 marker; and (3) are restricted by class I HLA transplantation antigens in their cytotoxic activities. We propose the hypothesis that interactions between HIV-specific CTL and infected macrophages induce major inflammatory reactions in seropositive patients.
人类免疫缺陷病毒（HIV）与获得性免疫缺陷综合征（AIDS）的发展有关。HIV感染会导致人类和猿类产生HIV特异性胸腺来源的（T）淋巴细胞。我们描述了一个实验系统，可对HIV特异性细胞毒性T淋巴细胞（CTL）进行定量和系统分析。功能性HIV特异性CTL是通过支气管肺泡灌洗（BAL）从患有淋巴细胞性肺泡炎的血清阳性患者的肺部获得的。这些肺泡CTL：（1）在自体而非异源条件下，在体外识别并杀死HIV感染的肺泡巨噬细胞；（2）与标准CTL一致，因为它们表达CD3和CD8表面标志物，但不表达CD4标志物；（3）其细胞毒性活性受I类HLA移植抗原限制。我们提出一个假设，即HIV特异性CTL与受感染巨噬细胞之间的相互作用会在血清阳性患者中引发主要炎症反应。
