Oghiso Y, Yamada Y, Shibata Y
Division of Comparative Radiotoxicology, National Institute of Radiological Sciences, Chiba, Japan.
Environ Health Perspect. 1992 Jul;97:159-61. doi: 10.1289/ehp.9297159.
Murine pulmonary alveolar macrophages (PAM) form macrophage colonies in vitro with colony-stimulating factors, which stimulate the clonal growth of radioresistant alveolar colony-forming cells (AL-CFC). The toxic effects of fibrogenic mineral dust particles on AL-CFC were investigated after intratracheal instillation into mice. Exposure to either crocidolite asbestos or silica (Min-u-sil) induced a significant depletion of AL-CFC as well as a decrease in PAM recovery compared to either untreated or titanium dioxide-exposed animals. Such effects were also noted with different doses (50-200 micrograms/animal) of instilled particles. The plating efficiency of AL-CFC was depleted in PAM exposed to fibrogenic particles in vitro, but not when exposed to nonfibrogenic titanium dioxide particles. These results indicate the toxic effects of fibrogenic dust particles on the clonal growth of PAM, cells which play a role in the clearance of inhaled particles from the lung and in subsequent pathologic processes.
小鼠肺泡巨噬细胞（PAM）在体外与集落刺激因子形成巨噬细胞集落，集落刺激因子刺激抗辐射肺泡集落形成细胞（AL-CFC）的克隆生长。将致纤维化矿物尘粒经气管内注入小鼠后，研究其对AL-CFC的毒性作用。与未处理或暴露于二氧化钛的动物相比，暴露于青石棉或二氧化硅（Min-u-sil）会导致AL-CFC显著减少以及PAM回收率降低。不同剂量（50 - 200微克/动物）的注入颗粒也观察到了这种效应。体外暴露于致纤维化颗粒的PAM中AL-CFC的接种效率降低，但暴露于非致纤维化二氧化钛颗粒时则未降低。这些结果表明致纤维化尘粒对PAM克隆生长具有毒性作用，PAM在从肺中清除吸入颗粒及后续病理过程中发挥作用。
