Chandran B, Tirawatnapong S, Pfeiffer B, Ablashi D V
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City.
J Med Virol. 1992 Aug;37(4):247-54. doi: 10.1002/jmv.1890370403.
Human herpesvirus 6 (HHV-6) prototype isolate GS is a newly identified lymphotropic herpesvirus and several subsequent herpes isolates were recognized as HHV-6 by their hybridization to a HHV-6(GS) DNA probe pZVH14. DNA restriction analysis and in vitro tropism studies show that HHV-6 isolates can be divided into two groups, designated group A and group B. Antigenic relationships among 15 HHV-6 isolates belonging to these two groups were examined using rabbit antibodies against HHV-6(GS) infected cells, 11 monoclonal antibodies against three glycoproteins and four non-glycoproteins of HHV-6(GS), and sera from 136 healthy adults. More than 20 polypeptides from all these isolates were immunoprecipitated by rabbit polyclonal antibodies against HHV-6(GS) infected cells. Reactivities of monoclonal antibodies segregated these isolates into the same two groups. Group A contains HHV-6(GS), HHV-6(U1102) from a Ugandan acquired immunodeficiency syndrome (AIDS) patient, and nine other HHV-6 isolates from various disorders. HHV-6(Z-29) from a Zairian AIDS patient, HHV-6(SF) isolated from the saliva of a human immunodeficiency virus (HIV)-infected individual, HHV-6(OK) from a child with exanthem subitum, and HHV-6(DC) from a leukopenia patient are in group B. Eighty-one percent of the sera showed similar antibody titer in immunofluorescence assay with group A HHV-6(GS) and group B HHV-6(Z-29) infected cells and 19% of the sera showed two- to four-fold antibody titer differences. The mobilities of many of the polypeptides immunoprecipitated from group A HHV-6(GS) and group B HHV-6(Z-29) infected cells were different and sera showed differences in the quantities and nature of polypeptides immunoprecipitated.(ABSTRACT TRUNCATED AT 250 WORDS)
人类疱疹病毒6型（HHV-6）原型毒株GS是一种新发现的嗜淋巴细胞疱疹病毒，随后的几种疱疹病毒分离株通过与HHV-6（GS）DNA探针pZVH14杂交而被确认为HHV-6。DNA限制性分析和体外嗜性研究表明，HHV-6分离株可分为两组，分别命名为A组和B组。使用针对HHV-6（GS）感染细胞的兔抗体、针对HHV-6（GS）三种糖蛋白和四种非糖蛋白的11种单克隆抗体以及136名健康成年人的血清，检测了属于这两组的15株HHV-6分离株之间的抗原关系。来自HHV-6（GS）感染细胞的兔多克隆抗体免疫沉淀了所有这些分离株的20多种多肽。单克隆抗体的反应性将这些分离株分为相同的两组。A组包含HHV-6（GS）、来自乌干达获得性免疫缺陷综合征（AIDS）患者的HHV-6（U1102）以及来自各种疾病的其他9株HHV-6分离株。来自扎伊尔AIDS患者的HHV-6（Z-29）、从人类免疫缺陷病毒（HIV）感染个体唾液中分离出的HHV-6（SF）、来自幼儿急疹患儿的HHV-6（OK）以及来自白细胞减少症患者的HHV-6（DC）属于B组。在免疫荧光试验中，81%的血清对A组HHV-6（GS）感染细胞和B组HHV-6（Z-29）感染细胞显示出相似的抗体滴度，19%的血清显示出2至4倍的抗体滴度差异。从A组HHV-6（GS）感染细胞和B组HHV-6（Z-29）感染细胞中免疫沉淀的许多多肽的迁移率不同，血清在免疫沉淀的多肽数量和性质上也存在差异。（摘要截短于250字）
