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酵母细胞色素c1前序列中一个19个氨基酸的疏水区域发生突变会阻止其分选至线粒体膜间隙。

Mutations in a 19-amino-acid hydrophobic region of the yeast cytochrome c1 presequence prevent sorting to the mitochondrial intermembrane space.

作者信息

Jensen R E, Schmidt S, Mark R J

机构信息

Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

出版信息

Mol Cell Biol. 1992 Oct;12(10):4677-86. doi: 10.1128/mcb.12.10.4677-4686.1992.

DOI:10.1128/mcb.12.10.4677-4686.1992
PMID:1328858
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360394/
Abstract

Most mitochondrial proteins destined for the intermembrane space (IMS) carry in their presequence information for localization to the IMS in addition to information for their import. By selecting for mutants in the yeast Saccharomyces cerevisiae that mislocalize an IMS-targeted fusion protein, we identified mutations in the IMS sorting signal of the cytochrome c1 protein. Amino acid substitutions or deletions in a stretch of 19 hydrophobic amino acids of the cytochrome c1 presequence resulted in accumulation of the intermediate form of the cytochrome c1 protein in the matrix. In some cases, the accumulated intermediate appeared to be slowly exported from the matrix, across the inner membrane to the IMS. Our results support the hypothesis that the cytochrome c1 precursor is normally imported completely into the matrix and then exported to the IMS.

摘要

大多数运往膜间隙（IMS）的线粒体蛋白，除了含有导入所需信息外，其前序列中还携带定位到IMS的信息。通过筛选酿酒酵母中使靶向IMS的融合蛋白定位错误的突变体，我们鉴定出了细胞色素c1蛋白的IMS分选信号中的突变。细胞色素c1前序列中一段19个疏水氨基酸的氨基酸替换或缺失，导致细胞色素c1蛋白的中间形式在基质中积累。在某些情况下，积累的中间体似乎从基质缓慢输出，穿过内膜到达IMS。我们的结果支持这样的假说，即细胞色素c1前体通常完全导入基质，然后输出到IMS。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a510/360394/f8d902d267ec/molcellb00133-0446-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a510/360394/d3941221ccaa/molcellb00133-0444-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a510/360394/20fa21e6b4fc/molcellb00133-0444-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a510/360394/9196903267e3/molcellb00133-0445-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a510/360394/f8d902d267ec/molcellb00133-0446-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a510/360394/d3941221ccaa/molcellb00133-0444-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a510/360394/20fa21e6b4fc/molcellb00133-0444-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a510/360394/9196903267e3/molcellb00133-0445-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a510/360394/f8d902d267ec/molcellb00133-0446-a.jpg

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本文引用的文献

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Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps.导入的线粒体蛋白细胞色素b2和细胞色素c1分两步进行加工。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):267-71. doi: 10.1073/pnas.79.2.267.
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Three-dimensional structure of membrane and surface proteins.膜蛋白和表面蛋白的三维结构。
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Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli.
响应促凋亡刺激时线粒体凋亡诱导因子的输出取决于线粒体外膜间隙的加工处理。
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Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane.Tim18p是线粒体内膜中Tim54p-Tim22p转位因子的一个新组分。
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The N-terminal hydrophobic region of the mature phosphate translocator is sufficient for targeting to the chloroplast inner envelope membrane.成熟磷酸转运体的N端疏水区域足以使其靶向叶绿体内膜。
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Targeting of cytochrome b2 into the mitochondrial intermembrane space: specific recognition of the sorting signal.细胞色素b2靶向进入线粒体膜间隙：分选信号的特异性识别。
EMBO J. 1993 Jun;12(6):2295-302. doi: 10.1002/j.1460-2075.1993.tb05883.x.
7
The signal that sorts yeast cytochrome b2 to the mitochondrial intermembrane space contains three distinct functional regions.将酵母细胞色素b2分选至线粒体膜间隙的信号包含三个不同的功能区域。
EMBO J. 1993 Jun;12(6):2303-11. doi: 10.1002/j.1460-2075.1993.tb05884.x.
8
MAS6 encodes an essential inner membrane component of the yeast mitochondrial protein import pathway.MAS6编码酵母线粒体蛋白质输入途径中一个必不可少的内膜成分。
J Cell Biol. 1993 Sep;122(5):1003-12. doi: 10.1083/jcb.122.5.1003.
9
Import of cytochrome b2 to the mitochondrial intermembrane space: the tightly folded heme-binding domain makes import dependent upon matrix ATP.细胞色素b2导入线粒体膜间隙：紧密折叠的血红素结合结构域使得导入依赖于基质ATP。
Protein Sci. 1993 Nov;2(11):1901-17. doi: 10.1002/pro.5560021112.
10
Loss of mitochondrial hsp60 function: nonequivalent effects on matrix-targeted and intermembrane-targeted proteins.线粒体热休克蛋白60功能丧失：对基质靶向蛋白和膜间隙靶向蛋白的不等效影响。
Mol Cell Biol. 1993 May;13(5):3050-7. doi: 10.1128/mcb.13.5.3050-3057.1993.
从信号肽中完全去除碱性氨基酸残基对大肠杆菌中脂蛋白分泌的影响。
J Biol Chem. 1983 Jun 10;258(11):7141-8.
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One-step gene disruption in yeast.酵母中的一步基因破坏
Methods Enzymol. 1983;101:202-11. doi: 10.1016/0076-6879(83)01015-0.
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Nucleic Acids Res. 1983 Mar 25;11(6):1645-55. doi: 10.1093/nar/11.6.1645.
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Import of proteins into mitochondria. The precursor of cytochrome c1 is processed in two steps, one of them heme-dependent.蛋白质导入线粒体。细胞色素c1的前体经过两步加工，其中一步依赖血红素。
J Biol Chem. 1982 Nov 10;257(21):13042-7.
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Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.蛋白质导入线粒体。细胞色素b2和细胞色素c过氧化物酶位于酵母线粒体的膜间隙中。
J Biol Chem. 1982 Nov 10;257(21):13028-33.
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A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
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Membrane-bound and water-soluble cytochrome c1 from Neurospora mitochondria.来自粗糙脉孢菌线粒体的膜结合型和水溶性细胞色素c1
Eur J Biochem. 1981 May;116(1):199-205. doi: 10.1111/j.1432-1033.1981.tb05319.x.
10
Two nuclear mutations that block mitochondrial protein import in yeast.两个阻断酵母线粒体蛋白导入的核突变。
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4819-23. doi: 10.1073/pnas.81.15.4819.