van Loon A P, Brändli A W, Schatz G
Cell. 1986 Mar 14;44(5):801-12. doi: 10.1016/0092-8674(86)90846-9.
Gene fusion experiments were used to identify signals that direct imported precursor proteins to specific intramitochondrial locations in yeast. The amino terminus of alcohol dehydrogenase III (ADHIII, a mitochondrial matrix enzyme) transported attached mouse dihydrofolate reductase (DHFR, a cytosolic enzyme) into the mitochondrial matrix. The presequence of cytochrome c1 (a mitochondrial inner membrane protein protruding into the intermembrane space) transported attached DHFR into the intermembrane space. The first half of the cytochrome c1 presequence, which resembles the ADHIII presequence, is a matrix-targeting sequence: it transported attached DHFR into the matrix. The second half of the cytochrome c1 presequence contains a stretch of 19 uncharged amino acids and may thus be a stop-transfer sequence. We conclude that intramitochondrial sorting involves matrix-targeting and stop-transfer sequences within the cleavable presequence.
基因融合实验被用于识别将导入的前体蛋白引导至酵母线粒体内特定位置的信号。醇脱氢酶III（ADHIII，一种线粒体基质酶）的氨基末端将附着的小鼠二氢叶酸还原酶（DHFR，一种胞质酶）转运至线粒体基质。细胞色素c1（一种突出于膜间隙的线粒体内膜蛋白）的前导序列将附着的DHFR转运至膜间隙。细胞色素c1前导序列的前半部分与ADHIII前导序列相似，是一个基质靶向序列：它将附着的DHFR转运至基质。细胞色素c1前导序列的后半部分包含一段19个不带电荷的氨基酸，因此可能是一个停止转移序列。我们得出结论，线粒体内分选涉及可裂解前导序列中的基质靶向和停止转移序列。
