Dulić V, Lees E, Reed S I
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Science. 1992 Sep 25;257(5078):1958-61. doi: 10.1126/science.1329201.
G1 cyclins control the G1 to S phase transition in the budding yeast, Saccharomyces cerevisiae. Cyclin E was discovered in the course of a screen for human complementary DNAs that rescue a deficiency of G1 cyclin function in budding yeast. The amounts of both the cyclin E protein and an associated protein kinase activity fluctuated periodically through the human cell cycle; both were maximal in late G1 and early S phases. Cyclin E-associated kinase activity was correlated with the appearance of complexes containing cyclin E and the cyclin-dependent kinase Cdk2. Thus, the cyclin E-Cdk2 complex may constitute a human G1-S phase-specific regulatory protein kinase.
G1 周期蛋白控制出芽酵母酿酒酵母中从 G1 期到 S 期的转变。细胞周期蛋白 E 是在筛选能够挽救出芽酵母中 G1 周期蛋白功能缺陷的人类互补 DNA 的过程中发现的。细胞周期蛋白 E 蛋白和相关蛋白激酶活性的量在人类细胞周期中呈周期性波动；两者在 G1 晚期和 S 早期均达到最大值。细胞周期蛋白 E 相关激酶活性与含有细胞周期蛋白 E 和细胞周期蛋白依赖性激酶 Cdk2 的复合物的出现相关。因此，细胞周期蛋白 E-Cdk2 复合物可能构成一种人类 G1-S 期特异性调节蛋白激酶。
