Galvin R J, Ramp W K, Lenz L G, Pierce W M
Department of Pharmacology & Toxicology, University of Louisville, KY 40292.
Toxicol Lett. 1992 Sep;62(2-3):301-10. doi: 10.1016/0378-4274(92)90034-h.
The effects of smokeless tobacco extract (STE) and various constituents of STE on prolyl hydroxylase activity were determined using enzyme extracted from chick embryos. STE inhibited prolyl hydroxylase activity in a concentration-dependent manner, but nicotine and anabasine had essentially no effect. Enzymatic activity was inhibited by zinc, but not by the other inorganic elements in STE; however, the zinc concentration in STE was not high enough to produce the observed inhibition. The inhibition by STE was diminished by increasing concentrations of 2-oxoglutarate, but not by increasing concentrations of other cofactors. Thus, STE contains an inhibitor of prolyl hydroxylase which may be competitive with 2-oxoglutarate.
使用从鸡胚中提取的酶，测定了无烟烟草提取物（STE）及其各种成分对脯氨酰羟化酶活性的影响。STE以浓度依赖性方式抑制脯氨酰羟化酶活性，但尼古丁和新烟草碱基本没有影响。酶活性受到锌的抑制，但不受STE中其他无机元素的抑制；然而，STE中的锌浓度不足以产生观察到的抑制作用。增加2-氧代戊二酸的浓度可减弱STE的抑制作用，但增加其他辅因子的浓度则不能。因此，STE含有一种脯氨酰羟化酶抑制剂，它可能与2-氧代戊二酸具有竞争性。
