Jimenez-Jativa S, Núñez de Castro I, Morata P
Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Malaga, España.
Biochem Int. 1992 Aug;27(5):923-9.
Fructose 1,6 bisphosphatase activity was measured in rat serum. The activity of the enzyme presented the following characteristics: pH optimum alkaline, between 8-8.5 and inhibited by AMP. The activity was measured in different experimental situations, such as streptozotocin diabetes, hepatocellular injury produced by carbon tetrachloride (CCl4), and bile-duct ligation. In diabetic rat, serum activity increased 2-fold with respect to the control values. In animals treated with CCl4 the activity increased 10-14 fold. On the contrary bile duct ligation decreased activity according to the cholestasis time. The results obtained in this study show that fructose 1,6 bisphosphatase is an enzyme measurable in serum, which changes according to different situations of liver cell injury.
在大鼠血清中测量了1,6-二磷酸果糖酶的活性。该酶的活性呈现出以下特征：最适pH呈碱性，介于8至8.5之间，且受AMP抑制。在不同的实验情况下测量了该活性，如链脲佐菌素诱导的糖尿病、四氯化碳（CCl4）引起的肝细胞损伤以及胆管结扎。在糖尿病大鼠中，血清活性相对于对照值增加了2倍。在用CCl4处理的动物中，活性增加了10至14倍。相反，胆管结扎会根据胆汁淤积时间降低活性。本研究获得的结果表明，1,6-二磷酸果糖酶是一种可在血清中测量的酶，其活性会根据肝细胞损伤的不同情况而变化。
