Wyrebowska J, Jerzykowski T
J Toxicol Environ Health. 1980 May;6(3):613-20. doi: 10.1080/15287398009529879.
Studies of aminopropanol dehydrogenase have shown no enzyme activity or very little activity in rat serum and in blood serum of healthy humans. After a single ip dose of CCl4 two enzymatic forms of aminopropanol dehydrogenase, reacting at different pH optima (alkaline and acid), appeared in the blood serum of rats. MFaximum enzyme activity at the alkaline pH appeared about 24 h after CCl4 administration in serum from young rats and after about 12 h in serum from mature rats. Activity values were about 90-150 mumol NAD reduced in 1 min per 1 l serum. Some biochemicl properties of the enzyme reacting at the alkaline pH optimum were studied. Aminopropanol dehydrogenase activity in serum of rats with acute CCl4 poisoning was determined by following the increase in absorbance at 340 nm due to the production of NADH. This enzyme, appearing in the serum after CCl4 poisoning, may be of interest as a potential indicator enzyme for clinical diagnosis.
对氨基丙醇脱氢酶的研究表明，在大鼠血清和健康人类血清中未检测到酶活性或仅有极低的活性。单次腹腔注射四氯化碳后，大鼠血清中出现了两种具有不同最适pH值（碱性和酸性）反应的氨基丙醇脱氢酶酶形式。碱性pH值下的最大酶活性在给年轻大鼠注射四氯化碳后约24小时出现在血清中，给成熟大鼠注射后约12小时出现在血清中。活性值约为每1升血清每分钟还原90 - 150微摩尔烟酰胺腺嘌呤二核苷酸（NAD）。研究了在碱性最适pH值下反应的酶的一些生化特性。通过跟踪由于烟酰胺腺嘌呤二核苷酸（NADH）产生导致的340纳米处吸光度的增加，测定急性四氯化碳中毒大鼠血清中的氨基丙醇脱氢酶活性。这种在四氯化碳中毒后出现在血清中的酶，作为临床诊断的潜在指示酶可能具有重要意义。
