Norén I, Ramström G, Wallén P
Haemostasis. 1975;4(2):110-24. doi: 10.1159/000214094.
A modification of the fibrin plate method is presented. Plasminogen-free human fibrinogen and plasminogen purified by affinity chromatography have been used. Fibrin plates without and with a constant amount of plasminogen and with agarose as stabilizing medium were used for the estimation of plasmin and plasminogen activator activity. Activator activity could be demonstrated in sterile bile and saliva. When plasmin activity was present, estimations of plasminogen activator were approximate. The method is sensitive, small volumes of reagents and samples are needed. The error of the method is comparatively low and the reproducibility is good.
本文介绍了一种纤维蛋白平板法的改良方法。使用了无纤溶酶原的人纤维蛋白原和通过亲和层析纯化的纤溶酶原。以无纤溶酶原、含恒定纤溶酶原量且以琼脂糖为稳定介质的纤维蛋白平板来评估纤溶酶和纤溶酶原激活剂活性。在无菌胆汁和唾液中可证实激活剂活性。当存在纤溶酶活性时，纤溶酶原激活剂的评估是近似的。该方法灵敏，所需试剂和样品体积小。该方法误差相对较低且重现性良好。
