Nishi M, Hanashiro R, Mori S, Masuda K, Mochizuki M, Hondo R
Department of Ophthalmology, Escola Paulista de Medicina, São Paulo, Brazil.
Am J Ophthalmol. 1992 Nov 15;114(5):603-9. doi: 10.1016/s0002-9394(14)74491-5.
We used the polymerase chain reaction to detect the virus genome in ocular samples from patients with clinically diagnosed acute retinal necrosis. Four samples from four patients with acute retinal necrosis, and five samples from three patients with other ocular diseases (sarcoidosis, rhegmatogenous retinal detachment, and epiretinal membrane of unknown origin) were evaluated. The samples consisted of aqueous humor, vitreous, or subretinal fluid. Primers were specific for varicella-zoster virus, herpes simplex virus, or cytomegalovirus. The varicella-zoster virus genome was detected in three of the four samples from patients with acute retinal necrosis. Among these three positive samples, two had PstI-site-less point mutation, strains that have been described only in Japan and of low prevalence. Samples from patients with diagnoses other than acute retinal necrosis yielded negative results when varicella-zoster virus primer was used. No sample was positive for herpes simplex virus or cytomegalovirus primers.
我们采用聚合酶链反应检测临床诊断为急性视网膜坏死患者眼部样本中的病毒基因组。对4例急性视网膜坏死患者的4份样本以及3例患有其他眼部疾病（结节病、孔源性视网膜脱离和不明来源的视网膜前膜）患者的5份样本进行了评估。样本包括房水、玻璃体或视网膜下液。引物针对水痘-带状疱疹病毒、单纯疱疹病毒或巨细胞病毒设计。在4例急性视网膜坏死患者的样本中，有3份检测到水痘-带状疱疹病毒基因组。在这3份阳性样本中，有2份存在无PstI位点的点突变，这种毒株仅在日本有报道且流行率较低。当使用水痘-带状疱疹病毒引物时，诊断为非急性视网膜坏死的患者样本结果均为阴性。没有样本对单纯疱疹病毒或巨细胞病毒引物呈阳性反应。
