Murphy S K, Oliver J D
Department of Biology, University of North Carolina, Charlotte 28223.
Appl Environ Microbiol. 1992 Sep;58(9):2771-5. doi: 10.1128/aem.58.9.2771-2775.1992.
Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days. Samples of oysters were homogenized and plated at intervals to determine the cell density of V. vulnificus and total aerobic population of bacteria present. At all temperatures, the numbers of V. vulnificus (both morphotypes) declined over the 10-day study period. The same observation was made with a lower inoculum of V. vulnificus. Identical experiments with shucked oysters showed a more rapid decrease in V. vulnificus. Identical experiments with shucked oysters showed a more rapid decrease in V. vulnificus to levels below limits of detection. Little change in the total bacterial counts was observed in shellstock oysters at any of the test temperatures, whereas incubation at the higher temperatures (17 and 22 degrees C) resulted in large increases in total counts in shucked oysters. These data suggest that temperature abuse of oysters may not be a factor in increasing the public health risk of V. vulnificus through raw oyster consumption. However, the data also suggest that even with proper storage, indigenous levels of V. vulnificus may remain sufficiently higher in shellstock oysters to produce infection in compromised hosts.
将含有TnphoA的创伤弧菌菌株的不透明和半透明形态型投喂给牡蛎，随后将牡蛎在0.5至22摄氏度的温度范围内储存10天。每隔一段时间对牡蛎样本进行匀浆并接种，以确定创伤弧菌的细胞密度和存在的细菌总需氧菌数。在所有温度下，创伤弧菌（两种形态型）的数量在10天的研究期内均下降。用较低接种量的创伤弧菌进行实验也得到了相同的观察结果。对去壳牡蛎进行的相同实验表明，创伤弧菌数量下降得更快。对去壳牡蛎进行的相同实验表明，创伤弧菌数量下降得更快，降至检测限以下。在任何测试温度下，带壳牡蛎的细菌总数几乎没有变化，而在较高温度（17和22摄氏度）下孵育会导致去壳牡蛎的总数大幅增加。这些数据表明，对牡蛎的温度滥用可能不是通过食用生牡蛎增加创伤弧菌公共卫生风险的一个因素。然而，数据还表明，即使妥善储存，带壳牡蛎中创伤弧菌的固有水平可能仍然足够高，足以在免疫力低下的宿主中引发感染。
