使用多黏菌素-多黏菌素B-纤维二糖琼脂从环境中分离创伤弧菌。
Use of colistin-polymyxin B-cellobiose agar for isolation of Vibrio vulnificus from the environment.
作者信息
Oliver J D, Guthrie K, Preyer J, Wright A, Simpson L M, Siebeling R, Morris J G
机构信息
Department of Biology, University of North Carolina, Charlotte 28223.
出版信息
Appl Environ Microbiol. 1992 Feb;58(2):737-9. doi: 10.1128/aem.58.2.737-739.1992.
Abstract
Colistin-polymyxin B-cellobiose agar was employed for the isolation of Vibrio vulnificus from shellfish. Isolates were examined phenotypically and with a gene probe and monoclonal antibody specific for V. vulnificus. Results indicated that colistin-polymyxin B-cellobiose agar is superior to both sodium dodecyl sulfate-polymyxin B-sucrose agar and thiosulfate-citrate-bile salts-sucrose agar in its ability to select and differentiate this species from background vibrios.
摘要
采用多黏菌素-多黏菌素B-纤维二糖琼脂从贝类中分离创伤弧菌。对分离菌株进行了表型分析，并使用针对创伤弧菌的基因探针和单克隆抗体进行检测。结果表明，在从背景弧菌中选择和区分该菌种的能力方面，多黏菌素-多黏菌素B-纤维二糖琼脂优于十二烷基硫酸钠-多黏菌素B-蔗糖琼脂和硫代硫酸盐-柠檬酸盐-胆盐-蔗糖琼脂。
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