Yoshida S, Ogawa M, Mizuguchi Y
Infect Immun. 1985 Feb;47(2):446-51. doi: 10.1128/iai.47.2.446-451.1985.
Colonies which varied in opacity were isolated from the four strains of Vibrio vulnificus. Opaque and translucent colonial types of the strains were distinguished from the corresponding parent strains. Variation in the opacity of colonies formed by each strain was accompanied by variation of capsular material formation, which was clarified by electron microscopy of the organisms stained with ruthenium red. The opaque-type colonies of the strains had capsular materials. On the other hand, three translucent-type colonies had no observable capsular materials, and one had incomplete capsular materials, in contrast to the corresponding opaque type. The corresponding opaque and translucent types of the strains were compared for points of virulence in mice and guinea pigs. By having capsular materials, the bacterial strains acquired resistance to serum bactericidal action, antiphagocytic activity, high lethality for mice, and strong invasiveness in the subcutaneous tissue of guinea pigs. Capsular materials of V. vulnificus were considered to be important for the expression of virulence.
从四株创伤弧菌中分离出了透明度不同的菌落。菌株的不透明和半透明菌落类型与相应的亲本菌株有所区别。每个菌株形成的菌落透明度变化伴随着荚膜物质形成的变化，这通过用钌红染色的生物体的电子显微镜观察得以阐明。菌株的不透明型菌落有荚膜物质。另一方面，与相应的不透明型相比，三个半透明型菌落没有可观察到的荚膜物质，一个有不完整的荚膜物质。比较了菌株相应的不透明型和半透明型在小鼠和豚鼠中的毒力点。通过拥有荚膜物质，细菌菌株获得了对血清杀菌作用的抗性、抗吞噬活性、对小鼠的高致死率以及在豚鼠皮下组织中的强侵袭性。创伤弧菌的荚膜物质被认为对毒力的表达很重要。
