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Nck的磷酸化反应涉及多种受体、佛波酯肉豆蔻酸酯乙酸盐和环磷酸腺苷。

Phosphorylation of Nck in response to a variety of receptors, phorbol myristate acetate, and cyclic AMP.

作者信息

Park D, Rhee S G

机构信息

Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892.

出版信息

Mol Cell Biol. 1992 Dec;12(12):5816-23. doi: 10.1128/mcb.12.12.5816-5823.1992.

DOI:10.1128/mcb.12.12.5816-5823.1992
PMID:1333046
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360521/
Abstract

The 47-kDa protein coimmunoprecipitated with phospholipase C (PLC)-gamma 1 by anti-PLC-gamma 1 monoclonal antibodies is proved to be Nck, a protein composed almost exclusively of one SH2 and three SH3 domains. Nck and PLC-gamma 1 are recognized by certain anti-PLC-gamma 1 monoclonal antibodies because Nck and PLC-gamma 1 share an epitope that likely is located in their SH3 domains. Nck is widely distributed in rat tissues, with an especially high level of expression in testes. The expression levels of Nck remains unchanged during the development of rat brain, whereas PLC-gamma 1 decreases during the same developmental period. Stimulation of A431 cells with epidermal growth factor elicits the tight association of Nck with the epidermal growth factor receptor and phosphorylation of Nck on both serine and tyrosine residues. The phosphorylation of Nck is also enhanced in response to stimulation of the nerve growth factor receptor in PC12 cells, the T-cell receptor complex in Jurkat cells, the membrane immunoglobulin M in Daudi cells, and the low-affinity immunoglobulin G receptor (Fc gamma RII) in U937 cells. The phosphorylation of Nck was also enhanced following treatment of A431 cells with phorbol 12-myristate 13-acetate or forskolin. These results suggest that Nck is a target for a variety of protein kinases that might modulate the postulated role of Nck as an adaptor for the physical and functional coordination of signalling proteins.

摘要

通过抗磷脂酶C（PLC）-γ1单克隆抗体与PLC-γ1共免疫沉淀的47 kDa蛋白被证明是Nck，一种几乎完全由一个SH2结构域和三个SH3结构域组成的蛋白质。Nck和PLC-γ1被某些抗PLC-γ1单克隆抗体识别，因为Nck和PLC-γ1共享一个可能位于其SH3结构域的表位。Nck广泛分布于大鼠组织中，在睾丸中的表达水平尤其高。在大鼠脑发育过程中，Nck的表达水平保持不变，而PLC-γ1在同一发育时期减少。用表皮生长因子刺激A431细胞会引发Nck与表皮生长因子受体的紧密结合以及Nck丝氨酸和酪氨酸残基的磷酸化。在PC12细胞中，对神经生长因子受体的刺激、在Jurkat细胞中对T细胞受体复合物的刺激、在Daudi细胞中对膜免疫球蛋白M的刺激以及在U937细胞中对低亲和力免疫球蛋白G受体（FcγRII）的刺激后，Nck的磷酸化也会增强。在用佛波醇12-肉豆蔻酸酯13-乙酸酯或福斯可林处理A431细胞后，Nck的磷酸化也会增强。这些结果表明，Nck是多种蛋白激酶的作用靶点，这些蛋白激酶可能会调节Nck作为信号蛋白物理和功能协调衔接子的假定作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/6f13e815a196/molcellb00135-0551-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/a66e6289cacf/molcellb00135-0548-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/b4ea04930bff/molcellb00135-0551-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/6f13e815a196/molcellb00135-0551-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/a66e6289cacf/molcellb00135-0548-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/b2a174b7bd93/molcellb00135-0548-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/7a8538e1317c/molcellb00135-0549-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/6fe76a136074/molcellb00135-0549-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/e0169df87233/molcellb00135-0550-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/e3e9ee7ea104/molcellb00135-0550-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/5e5308cf0399/molcellb00135-0551-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/b4ea04930bff/molcellb00135-0551-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44f9/360521/6f13e815a196/molcellb00135-0551-c.jpg

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