利用动基体DNA的限制性酶切分析对来自巴拉圭的克氏锥虫分离株进行鉴定

Characterization of Trypanosoma cruzi isolates from Paraguay, using restriction enzyme analysis of kinetoplast DNA.

作者信息

Mimori T, Maldonado M, Samudio M, De Arias A R, Moreno R, Sakamoto M

机构信息

Department of Parasitic Diseases, Kumamoto University Medical School, Japan.

出版信息

Ann Trop Med Parasitol. 1992 Jun;86(3):231-7. doi: 10.1080/00034983.1992.11812659.

DOI:10.1080/00034983.1992.11812659

PMID:1333178

Abstract

Eleven Paraguayan strains of Trypanosoma cruzi, from Chagas' disease patients and the bug vectors, were examined by restriction endonuclease analysis of kinetoplast DNA using Hae III, Msp I, Eco RI, HinfI, Taq I and Rsa I. Four schizodeme-profile groups were identified. Group 1 had much simpler profiles than groups 2, 3 and 4 and, although there were homogeneous profiles in the latter three groups, each group could be distinguished from the others. The profiles of group 1 could not be matched with any of the standard strains from Brazil, Chile and Columbia included in the schizodeme comparison. The profiles of groups 3 and 4 shared most features with those standards of the Brazilian Z2 zymodeme.

摘要

利用Hae III、Msp I、Eco RI、HinfI、Taq I和Rsa I对来自恰加斯病患者及虫媒的11株巴拉圭克氏锥虫进行动基体DNA的限制性内切酶分析。鉴定出四个裂殖体型谱组。第1组的谱型比第2、3和4组简单得多，后三组虽有均一的谱型，但每组均可与其他组区分开来。第1组的谱型与裂殖体型比较中纳入的巴西、智利和哥伦比亚的任何标准菌株均不匹配。第3和4组的谱型与巴西Z2酶型的那些标准谱型具有大多数共同特征。