de Lana M, Chiari C A, Chiari E, Morel C M, Gonçalves A M, Romanha A J
Departamento de Análises Clínicas, Escola de Farmácia, Universidade Federal, de Ouro Preto, MG, Brasil.
Parasitol Res. 1996;82(3):257-60. doi: 10.1007/s004360050106.
Two isolates of Trypanosoma cruzi were obtained from the patient Berenice, the first human case of Chagas' disease (Chagas 1909), when she was 55 and 71 years old, respectively. The isolates were characterized on the basis of their epimastigote-trypomastigote differentiation in liquid media and of the electrophoretic pattern of EcoR1 digestion products of kinetoplast DNA (k-DNA) minicircles (schizodeme) and isoenzyme patterns (zymodeme). Clear differences were found between the isolates, suggesting the occurrence of a heterogeneous population of T. cruzi in the infection of this patient.
从患者贝伦尼斯身上分离出两株克氏锥虫，她是恰加斯病的首例人类病例（查加斯，1909年），分离时她分别为55岁和71岁。根据它们在液体培养基中前鞭毛体-锥鞭毛体的分化情况、动质体DNA（k-DNA）小环的EcoR1消化产物的电泳图谱（裂殖体型）以及同工酶图谱（酶体型）对分离株进行了鉴定。在分离株之间发现了明显差异，这表明该患者感染的克氏锥虫群体存在异质性。
