Macina R A, Arauzo S, Reyes M B, Sanchez D O, Basombrio M A, Montamat E E, Solari A, Frasch A C
Instituto de Investigaciones Bioquimicas Fundación Campomar, Buenos Aires, Argentina.
Mol Biochem Parasitol. 1987 Aug;25(1):45-53. doi: 10.1016/0166-6851(87)90017-x.
Fifty-two isolates and several clones from Trypanosoma cruzi, the agent of Chagas' disease, were analyzed using cloned minicircles or total kinetoplast DNA as probes. Isolates were obtained from triatomines, guinea pigs and infected humans in the Central and Northern regions of Argentina and the North of Chile. 35% of all the randomly selected isolates could be identified with one cloned minicircle probe. This widely distributed T. cruzi group was detected on both sides of the Andes mountain range (Argentina and Chile) in Triatoma infestans as well as in human infections. Most of the other isolates could be grouped with four kinetoplast DNAs as probes, but their geographical distribution seems to be restricted as compared with the one mentioned above. These results confirm the heterogeneity of T. cruzi subspecies in nature and the usefulness of DNA probes to group them.
使用克隆的微小环或总动基体DNA作为探针，对52株来自恰加斯病病原体克氏锥虫的分离株和几个克隆进行了分析。分离株取自阿根廷中部和北部以及智利北部的锥蝽、豚鼠和受感染的人类。所有随机选择的分离株中有35% 可以用一种克隆的微小环探针进行鉴定。这个分布广泛的克氏锥虫群体在安第斯山脉两侧（阿根廷和智利）的侵扰锥猎蝽以及人类感染中均被检测到。其他大多数分离株可以用四种动基体DNA作为探针进行分组，但与上述群体相比，它们的地理分布似乎受到限制。这些结果证实了自然界中克氏锥虫亚种的异质性以及DNA探针用于对其进行分组的有效性。
