Cone R W, Hobson A C, Huang M L
Department of Laboratory Medicine, University of Washington Medical Center, Seattle 98195.
J Clin Microbiol. 1992 Dec;30(12):3185-9. doi: 10.1128/jcm.30.12.3185-3189.1992.
Polymerase chain reactions (PCR) may fail to react because the substrate DNA is absent (true negative) or because of inhibition of specific amplification (false negative). The use of positive controls can increase confidence in negative PCR results by ruling out failure due to inhibition as a cause of the lack of amplification products. This report describes the construction and application of coamplified positive controls for herpes simplex virus and human herpesvirus 6 amplifications. Herpes simplex virus and human herpesvirus 6 PCR products were modified to generate control PCR products in which the original probe sequences were replaced by a Drosophila probe sequence. Implementation of the coamplified controls increased our specimen throughput in comparison with the parallel control amplifications used previously. Clinical laboratories using PCR for diagnosis of infectious diseases may find positive controls particularly helpful for increasing confidence that negative amplifications represent truly negative specimens.
聚合酶链反应（PCR）可能无法发生反应，原因可能是底物DNA不存在（真阴性），也可能是特异性扩增受到抑制（假阴性）。使用阳性对照可以通过排除因抑制导致缺乏扩增产物这一原因，来增强对阴性PCR结果的信心。本报告描述了用于单纯疱疹病毒和人类疱疹病毒6扩增的共扩增阳性对照的构建和应用。对单纯疱疹病毒和人类疱疹病毒6的PCR产物进行了修饰，以生成对照PCR产物，其中原始探针序列被果蝇探针序列取代。与之前使用的平行对照扩增相比，共扩增对照的实施提高了我们的样本通量。使用PCR诊断传染病的临床实验室可能会发现，阳性对照对于增强阴性扩增代表真正阴性样本的信心特别有帮助。
