优化用于检测皮肤和黏膜上单纯疱疹病毒DNA的PCR阳性标准。
Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa.
作者信息
Magaret Amalia S, Wald Anna, Huang Meei-Li, Selke Stacy, Corey Larry
机构信息
Department of Laboratory Medicine, University of Washington, 600 Broadway, Suite 400, Seattle, WA 98122, USA.
出版信息
J Clin Microbiol. 2007 May;45(5):1618-20. doi: 10.1128/JCM.01405-06. Epub 2007 Feb 28.
Abstract
In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.
摘要
1997年，我们开发了一种用于检测单纯疱疹病毒（HSV）DNA的聚合酶链反应（PCR）检测方法。最近，我们基于研究标本和稀释研究确定了一个最佳阳性标准。我们发现，拭子标本中HSV DNA拷贝数为50拷贝/毫升的临界值，比我们之前的临界值低10倍，可将错误分类降至最低。
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