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本文引用的文献

1
Valacyclovir and acyclovir for suppression of shedding of herpes simplex virus in the genital tract.伐昔洛韦和阿昔洛韦用于抑制生殖道单纯疱疹病毒的脱落。
J Infect Dis. 2004 Oct 15;190(8):1374-81. doi: 10.1086/424519. Epub 2004 Sep 20.
2
Frequent reactivation of herpes simplex virus among HIV-1-infected patients treated with highly active antiretroviral therapy.在接受高效抗逆转录病毒治疗的HIV-1感染患者中，单纯疱疹病毒频繁再激活。
J Infect Dis. 2004 Aug 15;190(4):693-6. doi: 10.1086/422755. Epub 2004 Jul 13.
3
Once-daily valacyclovir to reduce the risk of transmission of genital herpes.每日一次服用伐昔洛韦以降低生殖器疱疹传播风险。
N Engl J Med. 2004 Jan 1;350(1):11-20. doi: 10.1056/NEJMoa035144.
4
Polymerase chain reaction for detection of herpes simplex virus (HSV) DNA on mucosal surfaces: comparison with HSV isolation in cell culture.用于检测黏膜表面单纯疱疹病毒（HSV）DNA的聚合酶链反应：与细胞培养中HSV分离法的比较
J Infect Dis. 2003 Nov 1;188(9):1345-51. doi: 10.1086/379043. Epub 2003 Oct 31.
5
Genital shedding of herpes simplex virus among men.男性生殖器单纯疱疹病毒脱落情况
J Infect Dis. 2002 Oct 15;186 Suppl 1:S34-9. doi: 10.1086/342969.
6
Reactivation of genital herpes simplex virus type 2 infection in asymptomatic seropositive persons.无症状血清阳性者中2型单纯疱疹病毒生殖器感染的再激活。
N Engl J Med. 2000 Mar 23;342(12):844-50. doi: 10.1056/NEJM200003233421203.
7
Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples.一种用于检测临床样本中单纯疱疹病毒DNA的高通量定量检测方法的开发。
J Clin Microbiol. 1999 Jun;37(6):1941-7. doi: 10.1128/JCM.37.6.1941-1947.1999.
8
Frequency of symptomatic and asymptomatic herpes simplex virus type 2 reactivations among human immunodeficiency virus-infected men.人类免疫缺陷病毒感染男性中2型单纯疱疹病毒有症状和无症状再激活的频率。
J Infect Dis. 1998 Dec;178(6):1616-22. doi: 10.1086/314486.
9
Herpes simplex virus shedding among human immunodeficiency virus-negative men who have sex with men: site and frequency of shedding.在与男性发生性行为的人类免疫缺陷病毒阴性男性中单纯疱疹病毒的排出情况：排出部位和频率
J Infect Dis. 1998 Oct;178(4):978-82. doi: 10.1086/515666.
10
Evaluation of a quantitative competitive PCR assay for measuring herpes simplex virus DNA content in genital tract secretions.用于测量生殖道分泌物中单纯疱疹病毒DNA含量的定量竞争性PCR检测方法的评估。
J Clin Microbiol. 1997 Mar;35(3):548-52. doi: 10.1128/jcm.35.3.548-552.1997.

优化用于检测皮肤和黏膜上单纯疱疹病毒DNA的PCR阳性标准。

Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa.

作者信息

Magaret Amalia S, Wald Anna, Huang Meei-Li, Selke Stacy, Corey Larry

机构信息

Department of Laboratory Medicine, University of Washington, 600 Broadway, Suite 400, Seattle, WA 98122, USA.

出版信息

J Clin Microbiol. 2007 May;45(5):1618-20. doi: 10.1128/JCM.01405-06. Epub 2007 Feb 28.

DOI:10.1128/JCM.01405-06
PMID:17329447
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1865863/
Abstract

In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.

摘要

1997年，我们开发了一种用于检测单纯疱疹病毒（HSV）DNA的聚合酶链反应（PCR）检测方法。最近，我们基于研究标本和稀释研究确定了一个最佳阳性标准。我们发现，拭子标本中HSV DNA拷贝数为50拷贝/毫升的临界值，比我们之前的临界值低10倍，可将错误分类降至最低。