Cloud S S, Lillehoj H S, Rosenberger J K
Delaware Agricultural Experiment Station, Department of Animal Science and Agricultural Biochemistry, University of Delaware, Newark 19716.
Vet Immunol Immunopathol. 1992 Nov;34(3-4):337-52. doi: 10.1016/0165-2427(92)90174-o.
The potential effect of chicken anemia agent (CAA) alone or in combination with infectious bursal disease virus (IBDV) on the immune system of young chickens was determined by measuring alterations in hematocrit values, lymphoid organ-to-body weight ratios and lymphoid cell concentrations at 4, 7, 10, 14, 17, 21, 28 and 42 days post-inoculation (PI). Lymphocyte subpopulations were identified and counted by flow cytometry using cell suspensions stained with monoclonal antibodies (Mabs) for panlymphocytes (K55), cytotoxic T-cells (CTLA3), T-helper cells (CT3), Ia-expressing cells (P2M11) and macrophages (P7). Chicken anemia agent induced a substantial but transient decrease in hematocrit value, thymus-to-body weight ratio and bursa-to-body weight ratio between 7 and 21 days PI corresponding to a generalized lymphocytopenia in the thymus, bursa and spleen. However, cytotoxic T-cell, T-helper cell and Ia-expressing cell concentrations increased in the bone marrow of birds inoculated with CAA alone or in combination with IBDV during the same time period. T-helper-to-cytotoxic T-cell ratios increased in the thymus and spleen during severe lymphocytopenia, indicating a selective decrease in cytotoxic T-cells. T-helper-to-cytotoxic T-cells ratios increased in the bone marrow, indicating a selective increase in T-helper cell concentrations. The increase in Ia-expressing cells in the bone marrow may be a reflection of increased number of activated T-cells which express Ia antigen. Infectious bursal disease virus alone induced a persistent depression of Ia-expressing cells in the bursa and the spleen and no measurable change in the bone marrow lymphocyte subpopulations. Chickens inoculated simultaneously with CAA and IBDV experienced clinical signs observed in chickens inoculated with each virus separately with a prolonged acute phase prior to recovery or mortality.
通过测量接种后（PI）第4、7、10、14、17、21、28和42天的血细胞比容值、淋巴器官与体重比以及淋巴细胞浓度的变化，确定鸡贫血因子（CAA）单独或与传染性法氏囊病病毒（IBDV）联合对幼鸡免疫系统的潜在影响。使用针对全淋巴细胞（K55）、细胞毒性T细胞（CTLA3）、辅助性T细胞（CT3）、Ia表达细胞（P2M11）和巨噬细胞（P7）的单克隆抗体（Mabs）染色的细胞悬液，通过流式细胞术鉴定并计数淋巴细胞亚群。鸡贫血因子在接种后7至21天导致血细胞比容值、胸腺与体重比以及法氏囊与体重比显著但短暂下降，这与胸腺、法氏囊和脾脏中的全身性淋巴细胞减少相对应。然而，在同一时期，单独接种CAA或与IBDV联合接种的鸡的骨髓中，细胞毒性T细胞、辅助性T细胞和Ia表达细胞的浓度增加。在严重淋巴细胞减少期间，胸腺和脾脏中的辅助性T细胞与细胞毒性T细胞的比率增加，表明细胞毒性T细胞选择性减少。骨髓中辅助性T细胞与细胞毒性T细胞的比率增加，表明辅助性T细胞浓度选择性增加。骨髓中Ia表达细胞的增加可能反映了表达Ia抗原的活化T细胞数量的增加。单独的传染性法氏囊病病毒导致法氏囊和脾脏中Ia表达细胞持续减少，并且骨髓淋巴细胞亚群没有可测量的变化。同时接种CAA和IBDV的鸡出现了分别接种每种病毒的鸡所观察到的临床症状，在恢复或死亡之前急性期延长。
