Wolff H, Panhans A, Zebhauser M, Meurer M
Dermatologische Klinik und Poliklinik, Ludwig-Maximilians-Universität München, Germany.
Fertil Steril. 1992 Dec;58(6):1260-2. doi: 10.1016/s0015-0282(16)55584-8.
Comparison of three methods for the detection of WBC in semen revealed a low concordance of positive test results. Among 557 semen samples, most positives were observed with the leukocyte esterase dipstick test (n = 95; 17.1%) followed by the peroxidase test (n = 51; 9.2%). There was little overlap between positives in the esterase-dipstick and the peroxidase method (29/117; 24.8%). With only 4 of 557 samples (0.7%), the PMN-elastase ELISA showed a surprisingly low incidence of positives. Because of lack of a gold standard, none of the three methods could be identified as superior. Because of its simplicity, specificity, and cost-effectiveness, the peroxidase method appeared most suited for clinical application.
三种精液白细胞检测方法的比较显示，阳性检测结果的一致性较低。在557份精液样本中，白细胞酯酶试纸检测法的阳性样本最多（n = 95；17.1%），其次是过氧化物酶检测法（n = 51；9.2%）。酯酶试纸法和过氧化物酶法的阳性结果几乎没有重叠（29/117；24.8%）。在557份样本中，仅有4份（0.7%）通过PMN-弹性蛋白酶ELISA检测呈阳性，阳性发生率出奇地低。由于缺乏金标准，无法确定这三种方法中哪种更优。过氧化物酶法因其简便、特异且具有成本效益，似乎最适合临床应用。
