Centre of Reproductive Medicine and Andrology, University of Münster, Germany.
Asian J Androl. 2010 Jan;12(1):111-4. doi: 10.1038/aja.2009.15.
The aim of this study was to increase the precision of assessment of the number of round cells observed in the peroxidase test for detection of seminal leukocytes (granulocytes). The dilution of semen was reduced and the volume of suspension examined was increased for semen samples containing between 0.6 and 6 million round cells per mL. A 1 + 5 (1:6) dilution was compatible with measurable peroxidase activity and a sufficiently clear background for cell assessment. At this dilution, and with assessment of all 18 grids on both sides of the Neubauer-improved counting chamber, only three of the 10 samples (nominal cell concentrations of 1.9 x 10(6) -3.3 x 10(6) mL(-1)) presented 400 round cells or more. As lower seminal dilutions were incompatible with easy detection of round cells or their peroxidase reaction product, it was not possible to provide precise measurements (sampling error 5%) of the suggested lower reference limit of 1 x 10(6) cells per mL. The results indicate that this poor precision of measuring 1 x 10(6) round cells per mL could explain the discrepant reports on the acceptability of the cut-off values for leukocytospermia. Such reference limits need to be established with statistically sound methods.
本研究旨在提高过氧化物酶试验检测精液白细胞（粒细胞）时观察到的圆形细胞数量评估的精确性。对于每毫升含有 0.6 至 600 万个圆形细胞的精液样本，减少精液稀释度并增加混悬液检查体积。1 + 5（1:6）稀释与可测量的过氧化物酶活性和细胞评估足够清晰的背景兼容。在这种稀释度下，通过评估新柏氏改良计数室两侧的所有 18 个网格，只有 10 个样本中的 3 个（名义细胞浓度为 1.9 x 10(6) -3.3 x 10(6) mL(-1))出现 400 个或更多个圆形细胞。由于较低的精液稀释度不便于轻易检测到圆形细胞或其过氧化物酶反应产物，因此无法对建议的 1 x 10(6) 个细胞/毫升的较低参考下限进行精确测量（采样误差为 5%）。结果表明，这种测量每毫升 1 x 10(6) 个圆形细胞的精度较差，可能解释了白细胞精子症的截止值可接受性的不一致报告。需要使用统计学上合理的方法来建立此类参考限值。
