Generation of T memory cells in one-way mixed lymphocyte culture. IV. Primary and secondary responses to soluble and insoluble membrane preparations and to ultraviolet-light-inactivated stimulator cells.

Neither normal CBA (H-2k) nor purified spleen T cells respond in vitro to soluble or insoluble membrane preparations or to ultraviolet-light-inactivated stimulator cells of the allogeneic DBA/2 (H-2d) strain. However, CBA spleen cells deprived of phagocytic cells show a slight proliferative response under these conditions. After being primed against mitomycin-blocked DBA/2 cells in one-way mixed lymphocyte culture, the secondary blast-derived T 'memory' cells display a good secondary blast (proliferative) response to both membrane antigens and to ultraviolet-light-inactivated stimulator cells. In addition to this, the secondary T lymphocytes--in contrast to nonprimed T cells--respond by cytotoxicity when ultraviolet-light-inactivated cells are used as the second stimulant.