可溶性膜蛋白诱导继发性细胞溶解性T淋巴细胞
The induction of secondary cytolytic T lymphocytes by solubilized membrane proteins.
作者信息
Mescher M, Sherman L, Lemonnier F, Burakoff S
出版信息
J Exp Med. 1978 Mar 1;147(3):946-51. doi: 10.1084/jem.147.3.946.
Abstract
Membrane-bound antigens responsible for induction of a secondary allogeneic murine cytolytic T-cell (CTL) response have been obtained in a soluble, biologically active form by deoxycholate solubilization of tumor cell plasma membranes. The active proteins are soluble by the criteria of both ultracentrifugation and gel filtration. The immunological specificity of the induced CTL and removal of the activity from solution by treatment with B6 anti-P815 (anti-H-2d) antiserum and Protein A-Sepharose demonstrate that the CTL-inducing activity is dependent upon solubilized major histocompatibility complex antigens.
摘要
通过用脱氧胆酸盐溶解肿瘤细胞质膜，已获得了负责诱导继发性同种异体小鼠细胞溶解T细胞（CTL）反应的膜结合抗原，其呈可溶的生物活性形式。根据超速离心和凝胶过滤的标准，这些活性蛋白是可溶的。诱导的CTL的免疫特异性以及用B6抗P815（抗H-2d）抗血清和蛋白A-琼脂糖处理从溶液中去除活性，证明CTL诱导活性取决于溶解的主要组织相容性复合体抗原。
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